Abstract: The present invention relates to primers for diagnosis of one or more kinds of cancers and a diagnosis kit comprising said primers. The primers are made from highly homologous areas of twelve MAGE subtypes and eight GAGE subtypes. The diagnostic kit comprising the said primers can detect six MAGE subtypes and eight GAGE subtypes respectively. The invention also includes methods for using the primers to diagnose the onset of cancer, to monitor the progression of disease, and to correlate the gene expression to the prognosis of disease.