Abstract: An improved transparent slide, and method of manufacture, the slide having a plurality of covered chambers defined between generally parallel and uniformly spaced sidewalls integral to the slide and having a channeled configuration for inducing a liquid specimen into the chambers, wherein the size of the chamber and the thickness of the side walls is significantly reduced from prior structures. An improved molding method adapted for producing the thinner slide sidewalls, the method including the use of a plurality of injection ports for delivering molten plastic to a mold and locating of a plurality of symmetrical dams in the mold that act to uniformly distribute and improve the flow of molten plastic near chamber forming portions of the mold.

Abstract: Microspores derived from flowering plants are dispersed in fluids to simulate leukocytes. Reconstituted body fluids utilized as standards in clinical examination of fluids such as urine contain a known quantity of microspores to simulate leukocytes when examined under a high power field. Microspores utilized to simulate leukocytes have a particle size of between 10 microns and 25 microns and are stained utilizing the same stains and techniques utilized for staining leukocytes. Microspores derived from the Paper Mulberry tree are highly preferred for use as leukocyte resembling particles.