Patents Assigned to Idaho Technology, Inc.
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Publication number: 20130171045Abstract: A device is provided for receiving a fluid sample. The device includes a fitment having a cavity formed therein. The cavity is provided under vacuum. The fitment also includes a port having a seal. The port is configured to provide fluid connection from an exterior surface of the fitment to the cavity upon opening of the seal. The device optionally includes a collapsible compartment coupled to the fitment and in fluid communication with the cavity.Type: ApplicationFiled: February 27, 2013Publication date: July 4, 2013Applicant: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.
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Publication number: 20130157376Abstract: Methods, devices, and systems are provided for calibrating heat sources of thermal cyclers.Type: ApplicationFiled: December 20, 2011Publication date: June 20, 2013Applicant: Idaho Technology, Inc.Inventor: Lyle Marion Nay
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Publication number: 20130157349Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.Type: ApplicationFiled: February 12, 2013Publication date: June 20, 2013Applicant: BioFire Diagnostics, Inc., f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc., f/k/a Idaho Technology, Inc.
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Publication number: 20130137172Abstract: Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include high density nucleic acid amplification and detection and immuno-PCR.Type: ApplicationFiled: January 28, 2013Publication date: May 30, 2013Applicant: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.Inventor: BioFire Diagnostics, Inc. f/k/a Idaho Technology, Inc.
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Publication number: 20120231522Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: ApplicationFiled: March 8, 2012Publication date: September 13, 2012Applicant: IDAHO TECHNOLOGY, INC.Inventors: Carl T. Wittwer, Gudrun Reed, Virginie Dujols, Luming Zhou
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Patent number: 8093002Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: GrantFiled: July 9, 2010Date of Patent: January 10, 2012Assignees: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl T. Wittwer, Gudrun Reed, Virginie Dujols, Luming Zhou
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Publication number: 20110238323Abstract: An experimental melting curve is modeled as a sum of a true melting curve and background fluorescence. A deviation function may be generated based upon the experimental melting curve data and a model of a background signal. The deviation function may be generated by segmenting a range of the experimental curve into a plurality of windows. Within each window, a fit between the model of the background signal and the experimental melting curve data may be calculated. The deviation function may be formed from the resulting fit parameters. The deviation function may include background signal compensation and, as such, may be used in various melting curve analysis operations, such as data visualization, clustering, genotyping, scanning, negative sample removal, and the like. The deviation function may be used to seed an automated background correction process. A background-corrected melting curve may be further processed to remove an aggregation signal.Type: ApplicationFiled: May 14, 2010Publication date: September 29, 2011Applicants: Idaho Technology, Inc., The University of Utah Research FoundationInventors: Thomas Charles Robbins, Robert Andrew Palais, Carl Thomas Wittwer
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Patent number: 7803551Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: GrantFiled: August 11, 2009Date of Patent: September 28, 2010Assignees: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl T. Wittwer, Virginie Dujols
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Patent number: 7785776Abstract: Methods for analyzing a target nucleic acid are provided. A fluorescent label attached to a nucleic acid is incorporated into at least one strand of the target nucleic acid and the methods include monitoring change in fluorescence emission resulting from dissociation of the labeled strand of the amplification product from its complementary strand.Type: GrantFiled: May 8, 2003Date of Patent: August 31, 2010Assignees: Idaho Technology, Inc., University of Utah Research FoundationInventors: Carl T. Wittwer, Cameron Gundry, Richard David Abbott, Derek Allen David
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Patent number: 7670832Abstract: The present invention is directed to devices for performing PCR and monitoring the reaction of a sample comprising a nucleic acid and a fluorescent dye. Illustrative devices comprise a heat exchange component for heating and cooling the sample, a control device for repeatedly operating the heat exchange component to subject the sample to thermal cycling, an excitation source for optically exciting the sample to cause the sample to fluoresce, a photodetector for detecting temperature-dependent fluorescence levels from the sample, and a processor configured to record and process emissions from the fluorescent dye.Type: GrantFiled: August 15, 2005Date of Patent: March 2, 2010Assignees: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl T. Wittwer, Kirk M. Ririe
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Patent number: 7630837Abstract: The present invention is directed to a nucleic acid quantification kit and method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample. Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.Type: GrantFiled: August 29, 2002Date of Patent: December 8, 2009Assignees: Idaho Technology, Inc., University of Utah Research FoundationInventors: David J. Eyre, Randy P. Rasmussen, Brian E. Caplin, Wade R. Stevenson, Deepika Marine deSilva
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Publication number: 20090117553Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: ApplicationFiled: October 31, 2007Publication date: May 7, 2009Applicants: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl T. Wittwer, Luming Zhou, Virginie E. Dujols, Joseph A. Holden, Carlynn Willmore-Payne
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Patent number: 7456281Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: GrantFiled: July 13, 2006Date of Patent: November 25, 2008Assignee: Idaho Technology, Inc.Inventor: Virginie E. Dujols
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Patent number: 7387887Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: GrantFiled: April 20, 2004Date of Patent: June 17, 2008Assignees: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl T Wittwer, Luming Zhou, Virginie E. Dujols, Joseph A. Holden, Carlynn Willmore-Payne
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Publication number: 20070020672Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: ApplicationFiled: July 13, 2006Publication date: January 25, 2007Applicant: Idaho Technology, Inc.Inventors: Carl Wittwer, Luming Zhou, Virginie Dujols, Joseph Holden, Carlynn Willmore-Payne
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Patent number: 7118867Abstract: The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/?l.Type: GrantFiled: November 19, 2002Date of Patent: October 10, 2006Assignees: Roche Diagnostics Corporation, Idaho Technology, Inc.Inventors: Karim Tabiti, Gisela Betzl, Richie Soong, Randy P. Rasmussen, Deepika Marine Desilva, John G. Ward, Hallegh Page Millward
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Publication number: 20050233335Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.Type: ApplicationFiled: April 20, 2004Publication date: October 20, 2005Applicants: University of Utah Research Foundation, Idaho Technology, Inc.Inventors: Carl Wittwer, Luming Zhou, Virginie Dujols, Joseph Holden, Carlynn Willmore-Payne
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Publication number: 20050226772Abstract: A device is provided for receiving a fluid sample. The device includes a fitment having a cavity formed therein. The cavity is provided under vacuum. The fitment also includes a port having a seal. The port is configured to provide fluid connection from an exterior surface of the fitment to the cavity upon opening of the seal. The device optionally includes a collapsible compartment coupled to the fitment and in fluid communication with the cavity.Type: ApplicationFiled: April 23, 2003Publication date: October 13, 2005Applicant: Idaho Technology, Inc.Inventors: Kirk Ririe, Brent Higginson, Abigail Bird