Patents Assigned to IDEC Pharmaceuticals Corp.
  • Novel method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
    Publication number: 20020192820
    Abstract: A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.
    Type: Application
    Filed: April 1, 2002
    Publication date: December 19, 2002
    Applicant: IDEC PHARMACEUTICALS Corp.
    Inventors: Mitchell R. Reff, Richard Spence Barnett, Karen Retta McLachlan
  • Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
    Patent number: 6413777
    Abstract: A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.
    Type: Grant
    Filed: June 30, 1999
    Date of Patent: July 2, 2002
    Assignee: IDEC Pharmaceuticals Corp.
    Inventors: Mitchell R. Reff, Richard Spence Barnett, Karen Retta McLachlan