Abstract: Methods useful for multiplex amplifying a plurality of loci or genetic systems comprise at least two loci. The at least two loci may be selected from the group consisting of D9S302, D22S683, D18S535, D3S2387, D4S2366, D5S1719 and D7S1804 loci. In another embodiment, preferably at least two loci comprise complex tandem repeat (CTR) sequences. The methods, kits, compositions and ladders disclosed herein are useful for analyzing human samples, as well as samples from other species. Analysis includes parentage, forensic, tissue origin, sample origin and genetic relatedness studies.