Abstract: Immunoassay methods employing capillary containers are provided. The immunoassays may be competitive or sandwich immunoassays, where fluorescently labeled conjugates are used. Sample suspected of containing analyte is combined with fluorescently labeled conjugate, as well as any additional reactants which may be required. At least a portion of the incubated sample is placed in a capillary container. The capillary container has, on at least one region of its inner surface, a binding member capable of complexing with the fluorescently labeled conjugate, either directly or indirectly. Fluorescently labeled conjugate not complexed to binding member is then washed from the capillary container. The complexed fluorescently labeled conjugate is detected by irradiating the capillary and measuring the emitted signal. The intensity of the emitted signal is indicative of the presence of analyte in the sample.

Abstract: A method for detecting boar taint in a pig sample is provided. Anti-skatole antibodies are combined in a reaction buffer with a pig sample to form a reaction mixture. After incubating, the amount of skatole-antibody complex formation is determined. In a particular embodiment, the method utilizes solid substrate-affixed antibodies in a competitive inhibition assay with an enzyme-labeled skatole analogue. Kits facilitating the method are also provided.