Patents Assigned to Illumina Cambridge Limited
  • Labelled nucleotides
    Patent number: 9121062
    Abstract: Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: September 1, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Shankar Balasubramanian, Colin Barnes, Xiaohai Liu
  • METHODS FOR SEQUENCING POLYNUCLEOTIDES
    Publication number: 20150225787
    Abstract: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
    Type: Application
    Filed: April 10, 2015
    Publication date: August 13, 2015
    Applicant: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Boutell Jonathan Mark
  • Method for Sequencing a Polynucelotide Template
    Publication number: 20150203910
    Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
    Type: Application
    Filed: December 17, 2014
    Publication date: July 23, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Geoffrey Paul Smith, Jonathan Mark Boutell, Colin Lloyd Barnes, Roberto Rigatti, Niall Anthony Gormley, David Bentley, Tobias William Barr Ost, Vincent Peter Smith, Graham John Worsley, Eric Hans Vermaas
  • METHOD FOR SEQUENCING A POLYNUCLEOTIDE TEMPLATE
    Publication number: 20150203911
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
    Type: Application
    Filed: March 31, 2015
    Publication date: July 23, 2015
    Applicant: Illumina Cambridge Limited
    Inventor: GEOFFREY PAUL SMITH
  • Preparation of templates for nucleic acid sequencing
    Patent number: 9085802
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: July 21, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Lloyd Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Dyes for labelling molecular ligands
    Patent number: 9085698
    Abstract: Cyanine dyes with improved fluorescence intensity and photostability.
    Type: Grant
    Filed: September 23, 2011
    Date of Patent: July 21, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Romanov, Carole Anastasi, Xiaohai Liu
  • END MODIFICATION TO PREVENT OVER-REPRESENTATION OF FRAGMENTS
    Publication number: 20150197789
    Abstract: The invention relates to a method of preparing a 5? and 3? modified library of template polynucleotides and also the use of the 5? and 3? modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5? and 3? modified library of template polynucleotides which have common sequences at their 5? ends and at their 3? ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from whence the 5? and 3? modified library is generated is greatly reduced or prevented.
    Type: Application
    Filed: March 24, 2015
    Publication date: July 16, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Helen Rachel Bignell
  • POLYNUCLEOTIDE MODIFICATION ON SOLID SUPPORT
    Publication number: 20150197799
    Abstract: The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface.
    Type: Application
    Filed: December 18, 2014
    Publication date: July 16, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Allen E. Eckhardt, Jonathan Mark Boutell
  • Using populations of beads for the fabrication of arrays on surfaces
    Patent number: 9079148
    Abstract: The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: July 14, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Geoffrey Paul Smith, Jonathan Mark Boutell
  • POLYMETHINE COMPOUNDS AND THEIR USE AS FLUORESCENT LABELS
    Publication number: 20150167073
    Abstract: The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Application
    Filed: March 2, 2015
    Publication date: June 18, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Methods for sequencing polynucleotides
    Patent number: 9029103
    Abstract: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: May 12, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Jonathan Mark Boutell
  • METHOD FOR RETAINING EVEN COVERAGE OF SHORT INSERT LIBRARIES
    Publication number: 20150119292
    Abstract: The invention relates to a method of preparing a library of template polynucleotides with uniform sequence representation and to use of a library of templates prepared using this method for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5? ends and at their 3? ends, which contains even representation of all the fragments present in a starting sample of nucleic acid before fragmentation. The invention is especially applicable to the preparation of short insert libraries, where the sample fragments are less than 150 base pairs in length.
    Type: Application
    Filed: December 3, 2014
    Publication date: April 30, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Niall Anthony Gormley, Melanie Anne Smith
  • Method for sequencing a polynucleotide template
    Patent number: 9017945
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: April 28, 2015
    Assignee: Illumina Cambridge Limited
    Inventor: Geoffrey Paul Smith
  • End modification to prevent over-representation of fragments
    Patent number: 9012184
    Abstract: The invention relates to a method of preparing a 5? and 3? modified library of template polynucleotides and also the use of the 5? and 3? modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5? and 3? modified library of template polynucleotides which have common sequences at their 5? ends and at their 3? ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from when the 5? and 3? modified library is generated is greatly reduced or prevented.
    Type: Grant
    Filed: February 7, 2007
    Date of Patent: April 21, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Niall Anthony Gormley, Helen Rachel Bignell
  • Polymethine compounds and their use as fluorescent labels
    Patent number: 8993784
    Abstract: The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: July 7, 2014
    Date of Patent: March 31, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Nikolai Nikolaevich Romanov, Xiaohai Liu
  • Methods of localizing nucleic acids to arrays
    Patent number: 8969258
    Abstract: Methods are disclosed relating to localizing nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.
    Type: Grant
    Filed: October 14, 2013
    Date of Patent: March 3, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Mark Edward Brennan Smith, Andrea Sabot, Isabelle Marie Julia Rasolonjatovo, Jean-Ernest Sohna Sohna, Adrian Martin Horgan, Harold Philip Swerdlow
  • TARGETED ENRICHMENT AND AMPLIFICATION OF NUCLEIC ACIDS ON A SUPPORT
    Publication number: 20150050657
    Abstract: Provided herein is a method of selecting target nucleic acids on a support. The method includes providing a plurality of beads each bead comprising one or more oligonucleotides, providing a support with a plurality of primers with a sequence complementary to at least a portion of the oligonucleotides on the beads, contacting the beads with the support wherein the oligonucleotides on the beads bind to the primers on the support, performing an extension reaction by extending the primers on the support to produce capture oligonucleotides, contacting the support comprising the capture oligonucleotides with the target nucleic acids, and extending the capture oligonucleotides bound to target nucleic acids to produce target extension products comprising a sequence complementary to at least a portion of the target nucleic acids. Optionally, the method further includes amplifying the target extension products.
    Type: Application
    Filed: May 28, 2013
    Publication date: February 19, 2015
    Applicant: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Jonathan Boutell
  • Method for sequencing a polynucleotide template
    Patent number: 8945835
    Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
    Type: Grant
    Filed: April 11, 2012
    Date of Patent: February 3, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Eric Hans Vermaas, Graham John Worsley, Jonathan Mark Boutell, Colin Lloyd Barnes, Roberto Rigatti, Niall Anthony Gormley, Geoffrey Paul Smith, Vincent Peter Smith, Tobias William Barr Ost, David Bentley
  • MODIFIED POLYMERASES FOR IMPROVED INCORPORATION OF NUCLEOTIDE ANALOGUES
    Publication number: 20150024463
    Abstract: The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3? position of the sugar moiety that are larger in size than the naturally occurring 3? hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
    Type: Application
    Filed: September 3, 2014
    Publication date: January 22, 2015
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Geoffrey Paul Smith, David Mark Dunstan Bailey, Raquel Maria Sanches-Kuiper, Harold Swerdlow, David James Earnshaw
  • METHOD OF NUCLEOTIDE DETECTION
    Publication number: 20140378323
    Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.
    Type: Application
    Filed: June 3, 2014
    Publication date: December 25, 2014
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Milan Fedurco, Anthony Romieu, Gerardo Turcatti