Abstract: In an example of the method, a functionalized coating layer is applied in depressions of a patterned flow cell substrate. The depressions are separated by interstitial regions. A primer is grafted to the functionalized coating layer to form a grafted functionalized coating layer in the depressions. A hydrogel is applied on at least the grafted functionalized coating layer.
Abstract: Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids.
Type:
Grant
Filed:
May 19, 2016
Date of Patent:
April 2, 2019
Assignee:
Ilumina, Inc.
Inventors:
Frank J. Steemers, Kevin L. Gunderson, Thomas Royce, Natasha Pignatelli, Igor Goryshin, Nicholas Caruccio