Abstract: Disclosed is a method for characterizing a sample by mass spectrometry imaging (MSI) according to which a spatial arrangement of at least one ion in the sample is characterized from imaging data associated with the ion, in terms of morphology and/or texture.

Abstract: The invention relates to a method for preparing a dilution range of at least one standard molecule for a tissue, whereby a tissue homogenate is used, to which the standard molecule is added, the resulting mixture being conditioned and sliced before the analysis of a slice of said tissue. The invention also relates to a method for detecting and quantifying at least one target molecule in at least one tissue using a standard range according to the invention.

Abstract: A method for processing a plurality of spectral datasets (J1-Jn) intended for being used by a molecular imaging method or a method for recording a plurality of spectral datasets (J1-Jn), each spectral dataset (J1-Jn) being defined by a set of spatial positions (Xi, Yj) each of which is associated with a molecular spectrum with at least two dimensions containing a set of molecular information (S(Xi, Yj)), the method including in particular the following steps: for each dataset (J1-Jn), cutting the molecular spectrum associated with each position (Xi, Yj) into a plurality of spectrum segments (T1-Tm); inserting the segments (T1-Tm) obtained for each position (Xi, Yj) of each dataset (J1-Jn) into a database (BDD); selecting in the database (BDD), following a request relating to molecular information of interest, the one or more segments (T1-Tm) containing the molecular information of interest; and selecting, within each segment (T1-Tm), the molecular information of interest.

Abstract: The invention relates to a method for evaluating whether a molecule of interest binds or is incorporated in at least one target tissue, comprising visualizing and comparing the distribution of the molecule of interest and of at least one control compound within the target tissue or on the target tissue surface of at least one animal that has previously received the molecule of interest and/or the control compound. Advantageously, the distribution of the molecule of interest is visualized and compared with the distribution of positive and negative compounds using mass spectrometry imaging on the surface of the target tissue.

Abstract: The invention relates to a method for identifying and quantifying by mass spectrometry imaging at least one target analyte in a tissue sample, comprising the following steps: a) depositing the tissue sample to be analyzed on a support; b) analyzing the sample by mass spectrometry imaging, so as to obtain the mass spectrum of the target analyte in said tissue sample; c) weighting a signal associated with the mass spectrum of the target analyte in said tissue sample by a tissue extinction coefficient (TEC) specific to the target analyte and to the tissue sample which is obtained by using a control tissue sample; and d) using the weighted signal of the target analyte to determine the quantity of target analyte in the tissue sample.

Abstract: The invention relates to a method for preparing a dilution range of at least one standard molecule for a tissue, whereby a tissue homogenate is used, to which the standard molecule is added, the resulting mixture being conditioned and sliced before the analysis of a slice of said tissue. The invention also relates to a method for detecting and quantifying at least one target molecule in at least one tissue using a standard range according to the invention.

Abstract: The invention relates to a method for identifying and quantifying by mass spectrometry at least one target molecule in a sample, comprising the following steps: a) depositing the sample to be analyzed on a support; b) analyzing the sample by mass spectrometry, so as to obtain the mass spectrum of the target molecule in said sample; c) weighting a signal associated with the mass spectrum of the target molecule in said sample by a extinction coefficient (TEC) specific to the target molecule and to the sample; and d) using the weighted signal of the target molecule to determine the quantity of target molecule in the sample.