Abstract: Provided are compositions that contain mammalian cells for use in detecting antibodies. The mammalian cells are modified such that they do not contain LEDGF protein. The mammalian cells are immobilized on a solid substrate. The compositions can also contain mammalian cells that contain the LEDGF protein. Methods for using the cell compositions in diagnostic approaches are included, as are kits for performing diagnostic tests.

Abstract: Provided are compositions that contain mammalian cells for use in detecting antibodies. The mammalian cells are modified such that they do not contain LEDGF protein. The mammalian cells are immobilized on a solid substrate. The compositions can also contain mammalian cells that contain the LEDGF protein. Methods for using the cell compositions in diagnostic approaches are included, as are kits for performing diagnostic tests.

Abstract: Provided are compositions and methods for diagnosis of celiac disease. The compositions include recombinant proteins that contain tissue transglutaminase and deamidated gliadin sequences. The gliadin sequences are repeated in the recombinant proteins. Also provided is a method for identifying an individual as having celiac disease based on the presence of antibodies in a sample from the individual, where the anti-bodies specifically recognize the recombinant protein, and identifying the individual as not having celiac disease based on an absence of antibodies that specifically recognize the recombinant protein.

Abstract: A flexible diagnostic workstation comprises is equipped to read label information on well strips of loaded well plates and on loaded reagent kit holders, automatically perform the pre-analytical steps of an immuno-assay which consists of a sequence of operations in accordance with scheduled test requirements for each microwell, read the results according to either a standard singleplex ELISA or multiplex test format as indicated by the well strip label, and report the results.