Abstract: Provided is a method of producing a mixture of pure feedstock-based native polyphenols from a feedstock. Contaminant polyphenols are first removed from an enzyme solution for converting feedstock to a product to produce a polyphenol reduced enzyme solution. The polyphenol reduced enzyme solution is combined with the feedstock and the feedstock is converted to a product and by-product. Heretofore, there has been no process available to reduce or remove the contaminant phenols introduced to the feedstock by commercial enzyme solutions. This method allows for the removal of contaminant phenols prior to introduction to the processing stream and subsequent harvesting of pure feedstock 6 based native polyphenols. The pure feedstock-based polyphenols are removed from the product or by-product to produce a pure mixture of feedstock-based polyphenols.
Abstract: Provided is a method for producing a concentrated oil-based polyphenol composition for human consumption. The unique combination of polyphenols in a biologic oil-based formulation may be administered for optimal effects on human platelets, lipids, and inflammatory markers. These physiologic changes have beneficial effects for human health and longevity.
Abstract: Provided is an enzyme immobilized on an enzyme. Also provided is a viscosified fracture fluid 1 formed by combining a solid proppant, a binding agent and an enzyme to form a proppant-immobilized enzyme 2, mixing the proppant-immobilized enzyme 2 with a second proppant 3 to form a heterogeneous proppant pack 4 and mixing said heterogeneous proppant pack 4 with a gelled liquid fracturing fluid 5 to form a viscosified fracture fluid 1 containing the heterogeneous proppant pack 4. The viscosified fracture fluid 1 containing the heterogeneous proppant pack 4 is pumped 6 into a subterranean zone 7 to form one or more fractures 8 therein. The proppant-immobilized enzyme 2 that is part of the heterogeneous proppant pack 4 has the dual purpose of propping open the subterranean fractures 8 and reducing the viscosity of the viscosified fracture fluid 1.
Abstract: A method of improving flow of liquids and gases from a subterranean fracture using a viscosified fracture fluid 1 formed by combining a solid proppant, a binding agent and an enzyme to form a proppant-immobilized enzyme 2, mixing the proppant-immobilized enzyme 2 with a second proppant 3 to form a heterogeneous proppant pack 4 and mixing said heterogeneous proppant pack 4 with a gelled liquid fracturing fluid 5 to form the viscosified fracture fluid 1 containing the heterogeneous proppant pack 4. The viscosified fracture fluid 1 containing the heterogeneous proppant pack 4 is pumped 6 into a subterranean zone 7 to form one or more fractures therein. The proppant-immobilized enzyme 2 that is part of the heterogeneous proppant pack 4 has the dual purpose of propping open the subterranean fractures and reducing the viscosity of the viscosified fracture fluid.
Abstract: A method of enhancing the intrinsic activity of an enzyme in a raw enzyme solution, the method comprising treating the raw enzyme solution with an effective amount of a purifying agent, most preferably, activated carbon, to effect the enhancement and provide an enzyme solution of enhanced activity. Preferred enzymes are amylase, glucoamylase, cellulase, xylanase, and all other group 3 hydrolases.
Type:
Grant
Filed:
March 11, 2004
Date of Patent:
February 22, 2011
Assignee:
Immortazyme Company
Inventors:
Bradley A. Saville, Mikhail I. Khavkine
Abstract: An improved method of making an immobilized enzyme comprising (a) treating an immobilization support with an aqueous solution comprising a cross-linking agent and polymeric aldehyde species and active centre species to produce a modified support; (b) isolating the modified support; (c) treating an enzyme solution with the modified support to produce the immobilized enzyme, the improvement comprising treating the aqueous solution of cross-linking agent with an effective amount of a purifying agent to reduce the amount of the polymeric aldehyde species and active centre species.
Type:
Grant
Filed:
March 11, 2004
Date of Patent:
December 25, 2007
Assignee:
Immortazyme Company
Inventors:
Bradley A. Saville, Mikhail I. Khavkine