Abstract: The present invention provides a hairpin ribozyme library having a randomized recognition sequence, packaged in a vector and operably linked to a promoter suitable for high level expression in a wide variety of cells. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.
Type:
Application
Filed:
July 2, 2003
Publication date:
May 20, 2004
Applicant:
Immusol, Incorporated
Inventors:
Jack R. Barber, Peter Welch, Soonpin Yei, Richard Tritz
Abstract: The present invention provides a hairpin ribozyme library having a randomized recognition sequence, packaged in a vector and operably linked to a promoter suitable for high level expression in a wide variety of cells. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.
Type:
Grant
Filed:
July 23, 1999
Date of Patent:
August 12, 2003
Assignee:
Immusol, Incorporated
Inventors:
Jack R. Barber, Peter Welch, Soonpin Yei, Richard Tritz
Abstract: GUC and GUA ribozymes which cleave HIV RNA are provided. The ribozymes cleave HIV RNA in vitro and in vivo. When the ribozymes are expressed in cells, they inhibit HIV replication in the cells.
Type:
Grant
Filed:
September 25, 1996
Date of Patent:
April 21, 1998
Assignee:
Immusol, Incorporated
Inventors:
Markley C. Leavitt, Richard Tritz, Elizabeth Duarte, Jack Barber, Mang Yu
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