Abstract: The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3?end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.
Type:
Grant
Filed:
April 10, 2006
Date of Patent:
August 2, 2011
Assignee:
In Situ RCP A/S
Inventors:
Jorn Erland Koch, Magnus Stougaard, Jakob Schwalbe Lohmann
Abstract: The present invention provides oligonucleotides and methods for efficient detection of target nucleic acids using rolling circle replication. In one aspect, the oligonucleotides of the invention are characteristic in that they can be circularised without an external ligation template. In another aspect, the oligonucleotides of the invention are characteristic in that they can generate a free 3?end of the target nucleic acid necessary for rolling circle replication. The oligonucleotides and detection methods of the invention are useful e.g. as research tool and for diagnosis.
Type:
Application
Filed:
April 10, 2006
Publication date:
February 25, 2010
Applicant:
IN SITU RCP A/S, C/O NOVI A/S
Inventors:
Jorn Erland Koch, Magnus Stougaard, Jakob Schwalbe Lohmann
Abstract: The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette.
Type:
Application
Filed:
April 10, 2006
Publication date:
February 4, 2010
Applicant:
IN SITU RCP A/S, C/O NOVI A/S
Inventors:
Jorn Erland Koch, Magnus Stougaard, Jakob Schwalbe Lohmann