Patents Assigned to Inalco S.p.A.
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Patent number: 9034844Abstract: The present invention relates to a process of synthesis of ?-sialyl oligosaccharides and in particular of 6?-sialyllactose and its salts comprising a step of coupling by Koenigs-Knorr reaction under conditions that allow its use on an industrial scale.Type: GrantFiled: April 6, 2010Date of Patent: May 19, 2015Assignee: INALCO S.P.A.Inventors: Giancarlo Tamerlani, Ilaria Lombardi, Debora Bartalucci, Andrea Danesi, Liana Salsini, Marco Manoni, Giovanni Cipolletti
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Patent number: 8227595Abstract: The present invention relates to a process for industrially producing tagatose starting from galactose and provides for chemical isomerisation in presence of XAlO2 and XOH (with X=Na, K) and microbiological purification of the residual galactose.Type: GrantFiled: March 9, 2010Date of Patent: July 24, 2012Assignee: Inalco S.p.A.Inventors: Luana Vagnoli, Silvia Giacomelli, Marco Manoni, Giovanni Cipolletti
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Publication number: 20120071441Abstract: The present invention relates to a process of synthesis of ?-sialyl oligosaccharides and in particular of 6?-sialyllactose and its salts comprising a step of coupling by Koenigs-Knorr reaction under conditions that allow its use on an industrial scale.Type: ApplicationFiled: April 6, 2010Publication date: March 22, 2012Applicant: INALCO S.P.A.Inventors: Giancarlo Tamerlani, Ilaria Lombardi, Debora Bartalucci, Andrea Danesi, Liana Salsini, Marco Manoni, Giovanni Cipolletti
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Patent number: 8124758Abstract: A process is described for the preparation of N-Acetyl-D-mannosamine monohydrate of formula (I) a specific intermediate in the synthesis of N-Acetyl-neuraminic acid, that is an important starting product for the synthesis of various pharmaceutically active products.Type: GrantFiled: May 18, 2007Date of Patent: February 28, 2012Assignee: Inalco S.p.A.Inventors: Giovanni Cipolletti, Giancarlo Tamerlani, Ilaria Lombardi, Debora Bartalucci
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Publication number: 20110245488Abstract: The present invention relates to a process for the synthesis of L-fucosyl di- or oligosaccharides and their novel 2,3,4-tri-O-benzyl-fucosyl synthetic intermediates derivatives of easy crystallization. In particular the present invention relates to a process applicable to industrial scale for the synthesis of 2?O-fucosyl lactose.Type: ApplicationFiled: December 18, 2009Publication date: October 6, 2011Applicant: INALCO S.P.A.Inventors: Liana Salsini, Marco Manoni, Giovanni Cipolletti
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Publication number: 20100261253Abstract: The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.Type: ApplicationFiled: July 8, 2008Publication date: October 14, 2010Applicants: BioTie Therapies Corp., Inalco S.p.A.Inventors: Markku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-Ping Li
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Patent number: 7687249Abstract: The present invention relates to a process for the preparation of sulphated glycosaminoglycans derived from N— acetylheparosan which comprises: a) N-deacetylation and N-sulphation of the N-acetylheparosan polysaccharide prepared from natural or recombinant bacterial strain, preferably K5 E. coli, b) enzymatic epimerization with the glucuronyl C5-epimerase enzyme, c) partial O-sulphation followed by a partial O-desulphation, d) partial 6-O-sulphation, e) N-sulphation and an intermediate step of controlled depolimerization characterised by the fact that both O-sulphations (O-sulphation and 6O-sulphation) are partial. Furthermore the invention relates to the products obtained according to the process which show a ratio between the anti-Xa activity and anti-IIa activity equal to or higher than 1 and to compositions comprising said products in combination with suitable and pharmaceutically acceptable excipients and/or diluent.Type: GrantFiled: July 7, 2004Date of Patent: March 30, 2010Assignee: Inalco S.p.A.Inventors: Marco Manoni, Liana Salsini, Jacopo Chini, Giovanni Cipolletti
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Patent number: 7399626Abstract: The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.Type: GrantFiled: April 11, 2005Date of Patent: July 15, 2008Assignees: BioTie Therapies Corp., Inalco S.p.A.Inventors: Markku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-Ping Li
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Patent number: 7361745Abstract: Herein described is a process for the preparation of 1-chloro-3,5-di-O-acyl-2-de-oxy-L-ribofuranoside derivatives of general formula (I) useful as intermediates in processes for preparing nucleotides of the L series having antiviral activityType: GrantFiled: November 10, 2004Date of Patent: April 22, 2008Assignee: Inalco S.p.A.Inventors: Giancarlo Tamerlani, Debora Bartalucci, Liana Salsini, Silvia Rapaccini
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Publication number: 20050181434Abstract: The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.Type: ApplicationFiled: April 11, 2005Publication date: August 18, 2005Applicants: BioTie Therapies Corp., Inalco S.p.A.Inventors: Markku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-ping Li
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Patent number: 6900311Abstract: The present invention refers to the preparation of O-sulphated, N-sulphatated or N-acetylated derivatives, both epimerised or non epimerised, of K5, K4, and optionally defructosilated K4 and K40 polysaccharides and to their use as antiinflammatory agents in chronic and acute inflammations.Type: GrantFiled: January 21, 2003Date of Patent: May 31, 2005Assignee: Inalco S.p.A.Inventors: Marco Manoni, Sandro Miletti, Giovanni Cipolletti, Rosanna Abbate, Maria Anna Gori
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Patent number: 6891036Abstract: The present invention relates to a new process in 3 steps starting from 2-C-methyl-D-ribopentono-1,4-lactone for the preparation of tetra-acyl ribofuranose derivatives of formula (I): useful in the synthesis of nucleotides.Type: GrantFiled: May 27, 2003Date of Patent: May 10, 2005Assignee: Inalco S.p.A.Inventors: Giancarlo Tamerlani, Liana Salsini, Ilaria Lombardi, Debora Bartalucci, Giovanni Cipolletti
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Patent number: 6887698Abstract: The invention is directed to a novel purified mouse C5-epimerase, fragments thereof, nucleic acids encoding the same and the recombinant production thereof. The invention is also directed to fragments of such epimerase, especially N-terminal fragments that are useful in fusion protein constructs to enhance the activity of recombinantly-produced heterologous epimerase enzymes.Type: GrantFiled: December 7, 2001Date of Patent: May 3, 2005Assignees: Biotie Therapies Corp., Inalco S.p.A.Inventors: Markku Jalkanen, Kamel El Darwish, Ulf Lindahl, Jin-ping Li
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Patent number: 6288044Abstract: Process for the preparation of O-sulfated K4, K5 and K40 polysaccharides useful for the treatment of tumoral, HIV-1 and coagulation pathologies and in cosmetic preparations, wherein the K4, K5 or K40 polysaccharide in the form of sodium salt is suspended in an aprotic solvent and directly submitted to the reaction of O-sulfation with a pyridine-sulphur trioxide or trimetylamine-sulphur trioxide adduct or with chlorosulfonic acid.Type: GrantFiled: July 23, 1999Date of Patent: September 11, 2001Assignee: INALCO S.p.A.Inventors: Giorgio Zoppetti, Pasqua Oreste, Giovanni Cipolletti
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Patent number: 6214124Abstract: Process for the preparation of lactulose wherein an aqueous solution or suspension of lactose is reacted with sodium aluminate in order to produce the isomerization of lactose and the mixture thus obtained is neutralized by treatment with gaseous CO2 under pressure, obtaining a suspension of aluminum hydroxide which can be easily separated from the lactulose solution.Type: GrantFiled: October 11, 1999Date of Patent: April 10, 2001Assignee: Inalco S.P.A.Inventors: Renato Carobbi, Giuseppe Bimbi, Giovanni Cipolletti
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Patent number: 6197943Abstract: Glycosaminoglycans having high antithrombotic activity in vitro, obtained by various kinds of glycosaminoglycans supersulfated by the preparation of the salt of an organic base of the starting supersaturated glycosaminoglycan, by partial solvolytic desulfation of said salt and N-resulfation of said partially desulfated product.Type: GrantFiled: September 20, 1999Date of Patent: March 6, 2001Assignee: Inalco S.p.A.Inventors: Benito Casu, Annamaria Naggi, Giangiacomo Torri
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Patent number: 6162797Abstract: Derivatives of the K5 polysaccharide having anticoagulant activity higher than heparin, obtained by a process including the steps of reacting: with an organic base a solution of K5 polysaccharide N-deacetylated, N-sulfated and epimerized at least to an iduronic acid content of 50% treating with a sulfating agent to obtain the N-resulfation of the possible N-desulfated groups.Type: GrantFiled: November 6, 1998Date of Patent: December 19, 2000Assignee: Inalco S.p.A.Inventors: Giorgio Zoppetti, Pasqua Oreste, Giovanni Cipolletti
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Patent number: 5958899Abstract: Process for the preparation of polysaccharides having a high iduronic acid content comprising:a) N-deacetylation of the polysaccharide K5 from E. coli or of the heparan sulfate or O-desulfation of heparin or heparan sulfate;b) N-sulfation of the product obtained from the stage a);c) epimerization in presence of the C5 epimerase enzyme;d) sulfation of at least some free hydroxy groups, wherein the stage c) is carried out in a reaction medium constituted by a classical buffer solution formed by HEPES, potassium chloride, EDTA and TRITON X-100 to which a suitable additive is added.Type: GrantFiled: April 12, 1996Date of Patent: September 28, 1999Assignee: Inalco S.p.A.Inventors: Giorgio Zoppetti, Pasqua Oreste, Giovanni Cipolletti
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Patent number: 5480491Abstract: The following description sets forth a new process for the preparation of .gtoreq.98.5% pure crystalline lactulose from commercially available aqueous syrups having the following composition: 50-70% by weight of lactulose, 3-9% by weight of lactose, 3-14% by weight of galactose, 4-7% by weight of other carbohydrates, the total content of carbohydrates different from lactulose being of from 10% to 30%.Type: GrantFiled: April 18, 1994Date of Patent: January 2, 1996Assignee: Inalco S.p.A.Inventor: Giuseppe Bimbi
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Patent number: 5011535Abstract: Stable boron resins of high selective absorbent power and their production process. Said boron resins consist of a polyacrylic matrix functionalized with quaternary ammonium groups, an epoxy group and phenylboric groups in accordance with general formula (I): ##STR1## in which P, R, R.sub.1, R.sub.2, R.sub.3, R.sub.4 and X.sup.- are as defined in the text. The resins are prepared by reacting an amine resin with an epihalohydrin and condensing the reaction product with a suitable boroxin. The resins of formula (I) can be used for separating sugars, in particular for purifying lactulose. They have greater selective power than currently known boron resins.Type: GrantFiled: May 24, 1990Date of Patent: April 30, 1991Assignee: Inalco S.p.A.Inventors: Renato Carobbi, Franco Innocenti