Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: An expression increasing element comprising a 36 nucleotide DNA sequence that can be used to increase the expression of genes, and in particular to increase the expression of endogenous plant genes, in plants is disclosed. Also disclosed are plants, plant parts, and commodity plant products comprising the expression increasing element along with related methods of using the expression increasing element and plants comprising the expression increasing element.

Abstract: Transgenic INHT30 soybean plants comprising modifications of the MON87708 soybean locus which provide for facile excision of the modified MON87708 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Compositions and methods related to Cas proteins, nucleic acids encoding the Cas proteins, and modified host cells comprising the Cas proteins and/or encoding nucleic acids are disclosed. Cas proteins are useful in a variety of applications. Cas proteins bind guide RNAs that in turn provide functional specificity to the Cas proteins, nucleic acids encoding the Cas guide RNAs, and modified host cells comprising the Cas guide RNAs and/or encoding nucleic acids. The Cas polypeptides and corresponding guide RNAs can be used in a variety of applications.

Abstract: Systems, methods, and compositions for providing improvements in plant gene-editing efficiency are provided.

Abstract: Examples of the disclosure describe systems and methods for identifying, quantifying, and/or characterizing plant stomata. In an example method, a first set of two or more images of a plant leaf representing two or more focal distances is captured via an optical sensor. A reference focal distance is determined based on the first set of images. A second set of two or more images of the plant leaf is captured via the optical sensor, including at least one image captured at a focal distance less than the reference focal distance, and at least one image captured at a focal distance greater than the reference focal distance. A composite image is generated based on the second set of images. The composite image is provided to a trainable feature detector in order to determine a number, density, and/or distribution of stomata in the composite image.

Abstract: The invention relates to novel maize plants, seeds and compositions, as well as improvements to maize plant breeding and methods for creating modifications in maize plant genomes. A transcriptional enhancer element which can increase the expression of both exogenous and endogenous maize genes is disclosed.

Abstract: The disclosure relates to improvements in methods for efficient editing of two or more endogenous tomato genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the tomato genome.

Abstract: The disclosure relates to precise modifications in wheat plant genomes including the heterologous integration of nucleic acid sequences that include a regulatory element at a predetermined locus. Also provided are wheat cells, wheat plants, wheat seeds, and processed wheat products comprising the modified wheat genomes.

Abstract: Provided herein are machine learning-based methods for assessing the combined impact of multiple genetic variants, as well as the uses of such methods for various applications, such as in synthetic biology, personalized medicine, agricultural breeding, and genetic engineering. Also provided herein are exemplar computer-readable storage media and electronic devices for performing such methods.

Abstract: Transgenic INIR20 soybean plants comprising modifications of the MON87701 soybean locus which provide for facile excision of the modified MON87701 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR11 maize plants comprising modifications of the MON89034 maize locus which provide for facile excision of the modified MON89034 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR19 soybean plants comprising modifications of the DAS81419 soybean locus which provide for facile excision of the modified DAS81419 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: The disclosure relates to improvements in methods for efficient editing of two or more endogenous soybean genes by insertion of one or more single stranded DNA donor molecules that are not homologous to the insertion sites in the soybean genome.

Abstract: Transgenic INHT31 soybean plants comprising modifications of the MON89788 soybean locus which provide for facile excision of the modified MON89788 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Plant cells and related systems, methods, and compositions for improving the capacity of the plant cells to regenerate embryogenic plant tissues, plant organs, and whole plants are provided. Such plant cells and related systems, methods, and compositions provide for increased expression of the endogenous morphoregulators BABYBOOM (ODP2) and/or WUSCHEL2 (WUS2).

Abstract: Transgenic INIR17 maize plants comprising an ecry3.1Ab expression cassette linked to a secondary nopaline synthase terminator element which lack a selectable marker gene and/or which comprise modifications that provide for facile excision of the INIR17 transgenic locus from the maize plant genome are provided. Genomic DNA of INIR17 transgenic plants, detection of INIR17 plants and products thereof, methods of making INIR17 plants, and use of INIR17 plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR4 maize plants comprising modifications of the DAS59122-7 maize locus which provide for facile excision of the modified DAS59122-7 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Transgenic INIR6 maize plants comprising modifications of the DP-4114 maize locus which provide for facile excision of the modified DP-4114 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.

Abstract: Compounds and related reagents, systems, methods, and compositions for increasing the frequency of homology directed repair (HDR) of target genomic sites with genome editing molecules are provided.