Abstract: Disclosed are an apparatus and a method for determining whether or not a biosensor comprising two working electrodes and one reference electrode is well manufactured, and for rapidly and accurately quantifying a specific substance contained in a biological sample. The method comprises the steps of: sequentially supplying the respective working electrodes with power supply voltage; sequentially detecting the amounts of current flowing in the respective working electrodes by virtue of the supplied power supply voltage; re-supplying the two working electrodes with power supply voltage after a predetermined time to redetect the amounts of current flowing in the respective working electrodes; reading concentrations corresponding to the amounts of current detected from a memory, and calculating an average value from the read concentrations; and checking whether or not the concentrations read from memory are within a predetermined critical range to display an error message or the calculated average value.
Abstract: A biosensor for quantifying a specific substance contained in a biological sample includes an electrically insulating base plate, a plurality of lead terminals formed on the base plate, a plurality of lead wires connected to the lead terminals, respectively, an electrode system including two working electrodes and one reference electrode connected to the lead wires, respectively, an insulating layer that insulates the electrodes, an enzyme reaction layer formed on the insulating layer and the electrodes, a spacer formed on the enzyme reaction layer so as to ensure a sufficient space that receives a sample, and a cover formed on the spacer. Further, the spacer has a sample introduction port opened at one side of the spacer, the cover has at least one slit for venting air existing in a sample receiving space defined by the spacer and the cover, and the slit extends to above the electrodes from one end of the cover.
Abstract: Disclosed is a biosensor for quantifying a specific substance contained in a biological sample comprising: an electrically insulating base plate; a plurality of lead terminals formed on the base plate; a plurality of lead wires connected to the lead terminals, respectively; an electrode system including two working electrodes and one reference electrode connected to the lead wires, respectively; an insulating layer for insulating the electrodes; an enzyme reaction layer formed on the insulating layer and the electrodes; a spacer formed on the enzyme reaction layer so as to ensure a sufficient space for receiving a sample; and a cover formed on the spacer, wherein the spacer has a sample introduction port opened at one side of the spacer, and the cover has at least one slit for venting air existing in a sample receiving space defined by the spacer and the cover, the slit extending to above the electrodes from one end of the cover.
Abstract: The present invention relates to a reference electrode for a potentiometric electrode system, characterized in that the junction in contact with a sample solution is enlarged and an ion-sensitive membrane containing an ion selective material as a protective membrane for the inner reference solution is employed, which comprises a substrate; a metal layer; an insoluble metal salt layer; an insulating film for insulating the metal layer from an aqueous solution; a hydrogel serving as an inner reference solution; the junction on the hydrogel contacting with a sample solution; the ion-sensitive, protective membrane formed over all surface areas of the hydrogel, except for the junction, to separate the sample solution from the inner reference solution or act as an ion-sensing membrane upon the contamination of the junction, whereby the operational abnormality caused by junction contamination may be easily checked and fast activation may be achieved.
Type:
Grant
Filed:
July 12, 2001
Date of Patent:
April 29, 2003
Assignee:
Infopia Co., Ltd.
Inventors:
Jae Ho Shin, Sung Dong Lee, Hakhyun Nam, Geun Sig Cha, Byeong Woo Bae