Abstract: A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.
Type:
Application
Filed:
December 29, 2023
Publication date:
May 2, 2024
Applicant:
INGURAN, LLC
Inventors:
Peter Kiesel, Todd Karin, Kenneth Michael Evans
Abstract: An optical arrangement receives output light emanating from an object disposed within a fluid column that crosses an optical refraction boundary of the fluid column between the object and the optical arrangement. The optical arrangement modifies the output light such that the modified output light has an intensity that is more uniform than the output light.
Type:
Application
Filed:
September 15, 2023
Publication date:
January 4, 2024
Applicant:
INGURAN, LLC
Inventors:
Alex Hegyi, Peter Kiesel, Kenneth M. Evans
Abstract: A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.
Type:
Grant
Filed:
January 19, 2023
Date of Patent:
January 2, 2024
Assignee:
INGURAN, LLC
Inventors:
Peter Kiesel, Todd Karin, Kenneth Michael Evans
Abstract: A method of manufacturing a nozzle assembly may include the step of over molding a nozzle housing, or a portion of a nozzle housing, onto at least one nozzle component, such as an injection tube. Nozzle assemblies and flow cytometers incorporating nozzle assemblies may include any combination of straight smooth injection tubes, improved features for securing a nozzle assembly, improved features for debubbilng a nozzle assembly, and aggressive orienting geometries. A method of sorting cells may include the step of magnetically coupling a nozzle assembly with a flow cytometer.
Type:
Application
Filed:
April 27, 2023
Publication date:
November 30, 2023
Applicant:
Inguran, LLC
Inventors:
Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
Abstract: A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.
Abstract: An optical arrangement receives output light emanating from an object disposed within a fluid column that crosses an optical refraction boundary of the fluid column between the object and the optical arrangement. The optical arrangement modifies the output light such that the modified output light has an intensity that is more uniform than the output light.
Type:
Grant
Filed:
August 8, 2022
Date of Patent:
October 24, 2023
Assignee:
Inguran, LLC
Inventors:
Alex Hegyi, Peter Kiesel, Kenneth M. Evans
Abstract: The invention encompasses methods for increasing genetic progress in livestock including the use of genomic evaluation of embryonic or fetal DNA obtained from allantoic fluid or cells therein.
Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
Type:
Application
Filed:
April 13, 2023
Publication date:
September 28, 2023
Applicant:
Inguran, LLC
Inventors:
Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.
Type:
Application
Filed:
February 14, 2023
Publication date:
August 24, 2023
Applicant:
INGURAN, LLC
Inventors:
Johnathan Charles Sharpe, Nemanya Sedoglavich, Blair Morad, Donald Francis Perrault, JR.
Abstract: A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system.
Type:
Grant
Filed:
August 19, 2021
Date of Patent:
August 8, 2023
Assignee:
Inguran, LLC
Inventors:
Gary Durack, Jeffrey D. Wallace, Gary P. Vandre, Lon A. Westfall, Jeremy T. Hatcher, Niraj V. Nayak
Abstract: The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.
Abstract: A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.
Type:
Application
Filed:
January 19, 2023
Publication date:
June 29, 2023
Applicant:
Inguran, LLC
Inventors:
Peter Kiesel, Todd Karin, Kenneth Michael Evans
Abstract: A method of sorting cells including magnetically coupling a nozzle assembly to a flow cytometer in a predetermined orientation to form a fluid stream having a sheath fluid and a sample fluid. Particles within the sample fluid are oriented and then interrogated at an inspection zone to produces signals representative of emitted or reflected electromagnetic radiation. The electromagnetic radiation is analyzed to classify the cells and the cells are sorted according to their classification.
Type:
Grant
Filed:
March 26, 2020
Date of Patent:
June 6, 2023
Assignee:
Inguran, LLC
Inventors:
Kris Buchanan, Johnathan Charles Sharpe, Kenneth Michael Evans
Abstract: A method for magnetic cellular manipulation may include contacting a composition with a biological sample to form a mixture. The composition may include a plurality of particles. Each particle in the plurality of particles may include a magnetic substrate. The magnetic substrate may be characterized by a magnetic susceptibility greater than zero. The composition may also include a chargeable silicon-containing compound. The chargeable silicon-containing compound may coat at least a portion of the magnetic substrate. The biological sample may include cells and/or cellular structures. The method may also include applying a magnetic field to the mixture to manipulate the composition.
Abstract: The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells.
Type:
Grant
Filed:
December 9, 2019
Date of Patent:
May 23, 2023
Assignee:
Inguran, LLC
Inventors:
Juan Moreno, Gregg Bevier, John Dobrinsky
Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
Type:
Grant
Filed:
March 18, 2020
Date of Patent:
April 18, 2023
Assignee:
Inguran, LLC
Inventors:
Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
Abstract: The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques.
Abstract: The present invention relates to compositions comprising low sugar media, the methods of using these compositions to reduce trauma and stress on processed animal sperm, the resulting sperm and embryo products, and the methods of use of these products to increase the quality, quantity and viability of progeny and improved rates of births in animals.
Type:
Application
Filed:
April 15, 2022
Publication date:
March 30, 2023
Applicant:
Inguran, LLC
Inventors:
Thomas B. Gilligan, Ramakrishnan Vishwanath, Kilby Willenburg
Abstract: This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and/or sorting.
Type:
Grant
Filed:
September 20, 2019
Date of Patent:
February 28, 2023
Assignee:
Inguran, LLC
Inventors:
Johnathan Charles Sharpe, Nemanya Sedoglavich, Blair Morad, Donald Francis Perrault, Jr.