Abstract: The invention relates to a dual-recognition immunoassay for the detection of antibodies specific to a target antigen in a sample which comprises contacting said target antigen with a sample suspected of containing said antibodies specific to said target antigen under conditions allowing the formation of an antigen-antibody complex; adding a conjugate comprising said target antigen and a marker under conditions allowing the formation of an antigen-antibody-antigen/marker complex; and detecting said antigen-antibody-antigen/marker complex. The immunoassay can be used, among other applications, in the diagnosis of infections caused by pathogenic organisms with high sensitivity and specificity.
Type:
Application
Filed:
July 28, 2008
Publication date:
August 5, 2010
Applicant:
INMUNOLOGIA Y GENETICA APLICADA, S.A.
Inventors:
Carmen Vela Olmo, Angel Antonio Venteo Moreno, Antonio Jose Sanz Fernández
Abstract: The IBDV VP2 icosahedral superstructures are made up of 12 empty VP2 dodecahedral capsids, lacking genetic material. They have a size of approximately 60–68 nm in diameter and contain 720 IBDV VP2 subunits. These VP2 icosahedral superstructures can be used for vaccinal and diagnostic purposes.
Type:
Grant
Filed:
July 5, 2002
Date of Patent:
November 7, 2006
Assignee:
Inmunologia y Genetica Aplicada, S.A.
Inventors:
Jose Ruiz Caston, Jorge Martinez Torrecuadrada, Jose Francisco Rodriguez Aguirre, Jose Ignacio Casal Alvarez, Jose Luis Lopez Carrascosa, Narcis Saubi Roca
Abstract: The recombinant fusion proteins comprise the protein of the nucleocapsid of the porcine reproductive and respiratory syndrome virus (PRRSV) obtained both from a European isolate of PRRSV as well as from an American isolate, fused to a sequence of amino acids derived from the system chosen for the expression of said recombinant fusion protein, either directly or by means of a binding area formed by a small number of amino acids. These recombinant fusion proteins are suited for their use in the diagnosis of PRRSV.
Type:
Grant
Filed:
May 17, 2002
Date of Patent:
July 15, 2003
Assignee:
Inmunologia Y Genetica Aplicada, S. A.
Inventors:
Ma Jose Rodriguez Garcia, Antonio Sanz Fernandez, Jose Ignacio Casal Alvarez
Abstract: The recombinant fusion proteins comprise the protein of the nucleocapsid of the porcine reproductive and respiratory syndrome virus (PRRSV) obtained both from a European isolate of PRRSV as well as from an American isolate, fused to a sequence of amino acids derived from the system chosen for the expression of said recombinant fusion protein, either directly or by means of a binding area formed by a small number of amino acids. These recombinant fusion proteins are suited for their use in the diagnosis of PRRSV.
Type:
Application
Filed:
May 17, 2002
Publication date:
June 12, 2003
Applicant:
Inmunologia Y Genetica Aplicada, S.A.
Inventors:
Ma Jose Rodriguez Garcia, Antonio Sanz Fernandez, Jose Ignacio Casal Alvarez
Abstract: The recombinant fusion proteins comprise the protein of the nucleocapsid of the porcine reproductive and respiratory syndrome virus (PRRSV), obtained both from a European isolate of PRRSV as well as from an American isolates, fused to a sequence of amino acids derived from the system chosen for the expression of said recombinant fusion protein, either directly or by means of a binding area formed by a small number of amino acids. These recombinant fusion proteins are suited for their use in the diagnosis of PRRSV.
Type:
Grant
Filed:
June 29, 1999
Date of Patent:
October 22, 2002
Assignee:
Inmunologia Y Genetica Aplicada, S.A.
Inventors:
Maria Jose Rodriguez Garcia, Antonio Sanz Fernandez, Jose Ignacio Casal Alvarez