Abstract: Method for the production of a subunit vaccine against porcine parvovirus (PPV). The method is comprised of a first step wherein a recombinant protein VP2 of PPV is obtained by using the replication of a recombinant baculovirus wherein the gene corresponding to VP2 has been previously inserted in cells of a permissive host. The protein VP2 obtained in this invention has the capacity of forming empty chimeric capsids with high immunogenicity and can be provided as a vaccine formulation for protecting pigs against PPV infection. The recombinant baculovirus AcMNPV.pPPVEx8 expresses the VP2 of PPV in conditions making possible the formation of pseudo-viral capsids.
Type:
Grant
Filed:
January 27, 1993
Date of Patent:
March 12, 1996
Assignee:
Inmunologia Y Genetica, S.A.
Inventors:
Jose I. Casal Alvarez, Elena Cortes Valdes, Ana I. Ranz Casares, Carmen Vela Olmo, Kristian Dalsgaard