Abstract: The invention concerns methods for producing lymphocytic cells, as well as tools, reagents and kits useful for implementing same. More particularly, it concerns methods for preparing gamma delta T cells, adapted to industrial production of functional cells of pharmaceutical quality in large amounts. The invention also concerns methods for activating gamma delta T cells, devices adapted to said methods, as well as the resulting cell compositions and their human or animal gamma delta T cells, and can be used in pharmaceutics, therapeutics, experiments, cosmetics, industrial research among others.