Abstract: The invention relates to a method for fermentation production of DHA, in particular a method for producing DHA through solid culture and liquid fermentation of Schizochytrium. The method comprises steps of activation of a strain, preparation of a suspension of the strain, preparation of a primary seed, preparation of a secondary solid seed, enlarging cultivation of the secondary solid seed in a fermentor, collection of cells after fermentation, and extraction of DHA oil. With the fermentation method, the seed preparation method is improved, the seed enlarging technology is simplified, the period of seed culture and fermentation is shortened, and therefore the investment is reduced and the cost is saved; in addition, the solid culture medium is of eutrophy and rich in a plurality of growth factors, and is more beneficial to the growth of the seed; cells are strong in viability and good in synchronism in solid culture medium.
Type:
Grant
Filed:
December 30, 2013
Date of Patent:
June 26, 2018
Assignees:
Xiamen Kingdomway Group Company, Inner Mongolia Kingdomway Pharmaceutical Limited
Abstract: A fermentation method for producing coenzyme Q10 is provided, including stepwisly culturing of microbial strains capable of producing coenzyme Q10, wherein key promoting factors are added in each stage of culture, and in the stage of culture in a fermentor, dissolved oxygen feedback-fed batch culture technique is adopted to realize the feedback regulation of the production of coenzyme Q10, so as to improve the yield of coenzyme Q10.
Type:
Grant
Filed:
August 10, 2011
Date of Patent:
June 27, 2017
Assignees:
Inner Mongolia Kingdomway Pharmaceutical Limited, Xiamen Kingdomway Group Company
Inventors:
Yi Wu, Biqin Chen, Guanghuang Zhan, Youpao Zhu
Abstract: The invention relates to a method for fermentation production of DHA, in particular a method for producing DHA through solid culture and liquid fermentation of Schizochytrium. The method comprises steps of activation of a strain, preparation of a suspension of the strain, preparation of a primary seed, preparation of a secondary solid seed, enlarging cultivation of the secondary solid seed in a fermentor, collection of cells after fermentation, and extraction of DHA oil. With the fermentation method, the seed preparation method is improved, the seed enlarging technology is simplified, the period of seed culture and fermentation is shortened, and therefore the investment is reduced and the cost is saved; in addition, the solid culture medium is of eutrophy and rich in a plurality of growth factors, and is more beneficial to the growth of the seed; cells are strong in viability and good in synchronism in solid culture medium.
Type:
Application
Filed:
December 30, 2013
Publication date:
December 17, 2015
Applicants:
Xiamen Kingdomway Group Company, Inner Mongolia Kingdomway Pharmaceutical Limited
Abstract: A fermentation method for producing coenzyme Q10 is provided, including stepwisly culturing of microbial strains capable of producing coenzyme Q10, wherein key promoting factors are added in each stage of culture, and in the stage of culture in a fermentor, dissolved oxygen feedback-fed batch culture technique is adopted to realize the feedback regulation of the production of coenzyme Q10, so as to improve the yield of coenzyme Q10.
Type:
Application
Filed:
August 10, 2011
Publication date:
November 14, 2013
Applicants:
Xiamen Kingdomway Group Company, Inner Mongolia Kingdomway Pharmaceutical Limited
Inventors:
Yi Wu, Biqin Chen, Guanghuang Zhen, Youpao Zhu