Abstract: A magnetic substance separation device is configured for attracting magnetic substances in a sample within a sample container. The magnetic substance separation device includes a casing and at least one magnetic component assembly. The casing has at least one accommodation compartment. The at least one magnetic component assembly is disposed in the at least one accommodation compartment, and the at least one magnetic component assembly includes at least four cubic magnetic components. In addition, the at least four cubic magnetic components are linearly arranged with different magnetization directions, allowing magnetic field lines of the at least one magnetic component assembly to concentrate on one side, such that the at least one magnetic component assembly forms at least one strong magnetic surface on the casing. The at least one strong magnetic surface is configured to attract the magnetic substances in the sample within the sample container.