Abstract: A method for differentiating adult stem cells into a final tissue is described. The method utilizes adult stem cells as a primary material for cultivation of fat and muscle tissue, resulting in an easier induction potential to differentiate into the target cells. To induce the differentiation, transcription factors are used.
Abstract: Biocompatible macroporous microcarriers, including microcarrier beads, microspheres, capsules, microsponges, hydrogels and other matrix forms, appropriate for use in a shaking flask or bioreactor to culture cells are described herein that can be used to create an edible structure for consumption or research investigation. Biocompatible, macroporous microcarriers can be dissolved or remain in the final product. Biocompatible macroporous microcarriers are formed by saccharides that are cross-linked via chemical induction with agitated cryo-gelation. Cross-linked macroporous, saccharide-microcarriers are coupled to adherence factors that enable cell binding. Finally, the cells are attached to the microcarrier for proliferation.