Abstract: An oil and gas exploration method based on a microbial gene is provided, where samples are collected from shallow surface layers above a known oil well, a gas well, and a dry well in an exploration area, DNA is extracted and subjected to high-throughput sequencing (HTS), and a pattern map of a microbial community composition in the exploration area is established according to sequencing results; and characteristic microorganisms in surface soil above an oil/gas well in the exploration area are screened out according to the pattern map, then primers are designed according to attribute characters of the characteristic microorganisms, and samples throughout the exploration area are subjected to fluorescence quantitative polymerase chain reaction (PCR) to detect a number of the characteristic microorganisms.

Abstract: An oil and gas exploration method based on a microbial gene is provided, where samples are collected from shallow surface layers above a known oil well, a gas well, and a dry well in an exploration area, DNA is extracted and subjected to high-throughput sequencing (HTS), and a pattern map of a microbial community composition in the exploration area is established according to sequencing results; and characteristic microorganisms in surface soil above an oil/gas well in the exploration area are screened out according to the pattern map, then primers are designed according to attribute characters of the characteristic microorganisms, and samples throughout the exploration area are subjected to fluorescence quantitative polymerase chain reaction (PCR) to detect a number of the characteristic microorganisms.