Abstract: A device of the present invention for sterile sampling of culture liquor from a fermenter comprises a sampler (1) positioned on the fermenter (3) in the zone of stirring the culture liquor and a sterile sample collector (11). The sampler (1) is fashioned as a normally closed valve whose body (4) has a circular projection (8) on the side of contact with the sample collector (11). The sample collector (11) is made evacuated and has a normally closed valve (12) mounted on its neck, the body (13) of which on the side of contact with the sampler (1) is provided a circular groove (17) conjugated with the circular projection (8) of the sampler (1). Upon superposition and interaction of the latter is formed a sealed conduit (19) for the passage of culture liquor from the fermenter (3) to the sample collector (11).
Type:
Grant
Filed:
October 31, 1983
Date of Patent:
August 25, 1987
Assignee:
Institut Biokhimii I Fiziologii Mikroorganizmov Akademii Nauk SSSR
Inventors:
Jury V. Redikultsev, Jury G. Zanin, Alexandr N. Shkidchenko, Oleg P. Gorbunov, Mikhail G. Maximov, Taisia S. Chermenskaya
Abstract: The present invention is directed to an apparatus for taking samples from a ermenter having such a construction as to enable sterilization of the equipment for taking the sample simultaneously with the procedure of sample taking. The possibility of inadvertently contaminating a fluid being sampled is prevented. The apparatus comprises a sampler (1) provided at the inlet with a valve (3), a by-pass valve (27) mountable at the outlet of a fermenter (39), and a separating chamber (19) between the valves (3) and (27). The chamber (19) has a nipple (40) with a valve (28) for feeding a sterilizing agent to the sampler (1). An additional valve (7) is secured at the inlet of the sampler (1), a housing of the valve (3) functioning as a shut-off member of this valve (7). The chamber (19) is capable of reciprocating motion in the direction of travel of the shut-off members (4) and (6) and engagement therewith.
Type:
Grant
Filed:
March 23, 1984
Date of Patent:
November 4, 1986
Assignee:
Institut Biokhimii I Fiziologii Mikroorganizmov Akademii Nauk SSSR
Inventors:
Jury V. Redikultsev, Mikhail G. Maximov