Abstract: This method, for determining the local intensity (I0) of an acoustic field propagating in a target region of a soft solid, at a position located within said target region, includes at least the following steps: determining (102) a value of an ultrasound attenuation coefficient (?) of the soft body in the target region; determining (104) a value of the shear modulus (?) of the soft body in the target region; determining (106) a value of the speed of sound (c) in the target region of the soft body; and building (110), with the values determined in steps a), b) and c), a viscoelastic model (M) of a steady-state displacement induced by an acoustic field having a time invariant shape or a viscoelastic model of a difference between two steady-state displacements induced by an acoustic field having a time invariant shape.

Abstract: The disclosure pertains to the field of molecular means capable of binding to, and preferably of chelating, cGMP, appropriate for use in vitro or in vivo and preferably capable of targeting specific cellular compartments. The polypeptides of the disclosure comprise a chimeric construction derived from the N terminus part of PKG-I? and PKG-I?, and the two cGMP binding sites of the wild type PKG.