Abstract: The present disclosure diagnoses mycobacterium tuberculosis, not mycobacterium tuberculosis complex only. Specific target genes are selected from regions of difference of mycobacterium tuberculosis. After hybridization with labeling substances, sputum samples are processed through color developments separately for obtaining images to be analyzed automatically. Thus, the present disclosure rapidly diagnoses mycobacterium tuberculosis in the sputum samples with a simple operation and a low cost.
Type:
Application
Filed:
January 15, 2010
Publication date:
July 21, 2011
Applicants:
Fooyin University, Institute for Biotechnology and Medicine Industry
Abstract: The present invention relates to a probe for detecting Mycobacterium tuberculosis complex (MTBC) and Mycobacterium tuberculosis (MTB) from clinical specimens. The method mainly comprised two steps: (1) amplifying a target DNA of the clinical specimens through nested PCR; and (2) hybridizing to the target DNA with a gold nanoparticle probe. The presence of MTB or MTBC was detected after the absorbance at 525 nm was determined with a spectrophotometer during hybridization at a defined time. Direct observation on color changes can also be detected after a longer incubating time. The merits of this assay include being easy to operate, sophisticated detection equipment is not necessary, low cost, time saving, and, high sensitivity and specificity.
Type:
Application
Filed:
December 22, 2009
Publication date:
January 13, 2011
Applicant:
INSTITUTE FOR BIOTECHNOLOGY AND MEDICINE INDUSTRY