Abstract: Methods of detecting target bacteria are provided. In some embodiments, the methods comprise exposing a sample to a phage capable of infecting target bacteria, said phage comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments, the target bacteria comprise Listeria. In some embodiments, the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.
Type:
Grant
Filed:
March 31, 2016
Date of Patent:
April 30, 2019
Assignee:
Institute for Enviornmental Health, Inc.
Inventors:
Michael Sandor Koeris, Robert Patrick Shivers, Daniel Robert Brownell, Jason Wyatt Holder, Jayson Linn Bowers