Abstract: The infectious clone comprises: a) a complete copy of the complementary DNA (cDNA) to the genomic RNA of the turnip mosaic virus (TuMV), in the form of double stranded DNA, b) a transcription promoter sequence, c) a replicon and, optionally, d) a transcription termination or polyadenilation sequence. Viral vectors comprise an infectious clone modified to contain a gene or a heterologous gene fragment. Infectious viral clones and vectors are useful for basic research, in virology and for the expression of genes and epitopes of interest.
Type:
Grant
Filed:
April 3, 2000
Date of Patent:
May 21, 2002
Assignee:
Institute Nacional de Investigacion Y Tecnologia Agraria Y
Alimentaria (INIA)
Inventors:
Fernando Ponz Ascaso, Vicente Torres Pascual, Florentina Sanchez Sanchez, David Martinez Herrera