Abstract: The present invention provides a method for preparing yeast beta-D-glucan using a solubilization technology based on molecular assembly, comprising the following steps: (1) micro-fluidizing an enzymatic hydrolysate of yeast cell walls at 70 to 200 MPa, and then centrifuging to obtain a precipitate; (2) resuspending the precipitate obtained in step (1) with a ionic liquid, then dispersing to obtain a solution; wherein the ionic liquid is 1-ethyl-3-methylimidazolium acetate or 1-allyl-3-methylimidazolium chloride; (3) centrifuging the solution obtained in step (2), and then adding ethanol, centrifuging and collecting a precipitate; (4) resuspending the precipitate obtained in step (3) with water, then centrifuging and collecting a supernatant. Preferably, the method further comprises (5): spray drying the supernatant obtained in step (4) to obtain a yeast beta-D-glucan powder.
Type:
Grant
Filed:
September 4, 2015
Date of Patent:
October 9, 2018
Assignee:
Institute of Agro-Products Processing Science and Technology, CAAS
Inventors:
Qiang Wang, Hongzhi Liu, Li Liu, Aimin Shi, Hui Hu, Yanan Li, Weijing Lin, Yuquan Duan, Jie Gao, Xiaoyong Liu