Patents Assigned to Institute of Animal Science
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Patent number: 11767356Abstract: Provided are a canine parvovirus (CPV) nanobody CPV-VHH-E3 and application thereof, belonging to the technical field of immunology. The nanobody CPV-VHH-E3 includes heavy chain variable region with amino acid sequence as shown in SEQ ID NO: 1, and a nucleotide sequence of a gene encoding the nanobody CPV-VHH-E3 is shown in SEQ ID NO: 2. The present application constructs a nanobody immune library for CPV by phage-display technology, and obtains specific anti-CPV nanobody CPV-VHH-E3 by screening, which is verified to specifically bind CPV through experiments, and is applicable to develop a nanobody preparation for clinical diagnosis and treatment of CPV, providing a certain theoretical support for the application of nanobodies in the field of veterinary biological products.Type: GrantFiled: November 9, 2022Date of Patent: September 26, 2023Assignee: INSTITUTE OF ANIMAL SCIENCES OF CAASInventors: Hong Jia, Hongfei Zhu, Qianqian Feng, Zhaoyang Wang, Weifeng Yuan, Zhongbao Pang
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Publication number: 20230212694Abstract: Disclosed is an SNP marker related to wool traits of a fine-wool sheep, and a detection primer set, a detection method and use thereof, which belong to the technical field of molecular marker detection; the SNP marker is located at 133486008bp on sheep chromosome 3. There is an A/C base mutation at the site, which is significantly related to the wool traits of fine-wool sheep. The SNP marker of the present disclosure may be used in molecular marker assisted breeding of sheep. The molecular marker provided by the present disclosure is not limited by the age, sex, etc. of the sheep, may be used for fine-wool sheep breeding (even a sheep at birth may be accurately screened), and significantly promotes the breeding process of fine-wool sheep.Type: ApplicationFiled: June 28, 2021Publication date: July 6, 2023Applicant: Institute of Animal Sciences of Chinese Academy of Agricultural SciencesInventors: Yuehui Ma, Lin Jiang, Benmeng Liang, Yabin Pu
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Publication number: 20230192815Abstract: The present disclosure provides a canine parvovirus (CPV) nanobody CPV-VHH-H1 and use thereof, belonging to the technical field of immunology. A heavy-chain variable region sequence of the nanobody CPV-VHH-H1 has the amino acid sequence set forth in SEQ ID NO: 1; and a gene encoding the nanobody CPV-VPP-H1 has the nucleotide sequence set forth in SEQ ID NO: 2. In the present disclosure, a nanobody immune library of the CPV is constructed by a phage display technology, a specific anti-CPV nanobody CPV-VHH-H1 is obtained by screening, and it is verified by experiments that the nanobody may specifically bind to the CPV. The present disclosure is expected to develop a new nanobody preparation for use in clinical diagnosis and treatment of the CPV, and provides a certain theoretical reserve for applying the nanobody to the field of veterinary biological products.Type: ApplicationFiled: November 10, 2022Publication date: June 22, 2023Applicant: Institute of Animal Sciences of Chinese Academy of Agricultural SciencesInventors: HONG JIA, Hongfei ZHU, Weifeng YUAN, Shaohua HOU, Xiaoyu GUO, Yitong JIANG, Qianqian FENG, Zhaoyang WANG, Ting XIN
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Publication number: 20230167462Abstract: The present disclosure provides a recombinant adenovirus for expressing African swine fever virus (ASFV) EP153R-EP402R protein and a construction method thereof, and belongs to the technical field of genetic engineering. In the present disclosure, a recombinant adenovirus vector pAD-CMV-EGFP-EP153R-EP402R is obtained through a series of intermediate processes using a recombinant adenovirus shuttle vector pENTRE-EGFP-TOPO; the recombinant adenovirus vector is linearized to transfect AD293 cells, a recombinant virus is screened according to cytopathy formed by adenovirus infection, an adenovirus packaging process is achieved, and the recombinant adenovirus for expressing ASFV EP153R-EP402R protein is obtained, laying a foundation for the construction of a recombinant adenovirus vaccine for expressing the ASFV EP153R-EP402R protein.Type: ApplicationFiled: November 22, 2022Publication date: June 1, 2023Applicant: Institute of Animal Sciences of Chinese Academy of Agricultural SciencesInventors: HONG JIA, Hongfei ZHU, Xiaoyu GUO, Weifeng YUAN, Shuai CUI, Yang WANG, Ting Xin
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Patent number: 11542562Abstract: The invention relates to a single nucleotide polymorphism (SNP) marker related to a Chinese horse short stature trait. The SNP molecular marker is located at the 501th position of a sequence shown in SEQ ID NO.1, polymorphism is G/A, and the SNP marker corresponds to base pair 18,205,998 on chromosome 8 in a horse. The SNP marker related to the Chinese horse short stature trait and use thereof provided by the present invention have the following advantages that: (1) the molecular marker is not restricted by the age, sex and the like of Chinese horses, is used in early breeding of the Chinese horses, performs accurate screening immediately at birth, and significantly promotes the breeding process of dominant pony varieties of the Chinese horse; (2) a method for detecting SNP of a Chinese horse TBX3 gene is accurate, reliable, and easy to operate.Type: GrantFiled: September 2, 2020Date of Patent: January 3, 2023Assignee: INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Yuehui Ma, Lin Jiang, Xuexue Liu, Yabin Pu, Yanli Zhang
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Patent number: 11377647Abstract: The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.Type: GrantFiled: March 21, 2019Date of Patent: July 5, 2022Assignee: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMYInventors: Bin Yao, Huiying Luo, Jin Qiu, Tao Tu, Huoqing Huang, Yuan Wang, Yaru Wang, Yingguo Bai, XiaoYun Su, Kun Meng
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Publication number: 20210024908Abstract: The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.Type: ApplicationFiled: March 21, 2019Publication date: January 28, 2021Applicant: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Bin YAO, Huiying LUO, Jin QIU, Tao TU, Huoqing HUANG, Yuan WANG, Yaru WANG, Yingguo BAI, XiaoYun SU, Kun MENG
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Patent number: 10883085Abstract: The present invention relates to porcine reproductive and respiratory syndrome virus (PRRSV) vaccines in a field of veterinary biological products, and more particularly to a PRRSV SX-105 strain having a deposit number of CGMCC No. 9906, and a use thereof in preparing PRRSV live vaccines. PRRSV SX-1 original strains are strongly pathogenic to swine. A PRRSV SX-105 attenuated strain shows safety and good immunogenicity on the swine. The PRRSV live vaccines, prepared from the PRRSV SX-105 strain, are safe and reliable, and able to generate strong immunity after immunization. The PRRSV live vaccines significantly decrease morbidity and mortality in inoculated swine groups, and have immunization effects reaching and slightly better than conventional commercial vaccines on the market, showing advantages to compete with the same kind of products around the world. The PRRSV live vaccines are capable of effectively preventing PRRS epidemic and transmission, and has broad application prospects.Type: GrantFiled: January 29, 2015Date of Patent: January 5, 2021Assignee: Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural SciencesInventors: Jiaqiang Wu, Jiang Yu, Yuyu Zhang, Xiwang Zhu, Yijun Du, Jun Li, Xiaoyan Cong, Jinbao Wang
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Patent number: 10485584Abstract: Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.Type: GrantFiled: June 8, 2017Date of Patent: November 26, 2019Assignees: Lekarska fakulta v Plzni, Univerzita Karlova (Faculty of Medicine in Pilsen, Charles University), The Curators of the University of Missouri, Chonbuk National University, Division of Biotechnology, College of Environmental and Bioresources, Ceska zemedelska univerzita v Praze, Institute of Animal ScienceInventors: Jan Nevoral, Young-Joo Yi, Katerina Zamostna-Adamkova, Jaroslav Petr, Peter Sutovsky, Milena Kralickova
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Publication number: 20190042705Abstract: The invention relates to a realization method for computer-aided screening of target aptamers and small molecule compounds, which is realized by adopting a molecular docking technology-based reverse virtual screening algorithm and comprises the steps of generating random unrepeated sequences with an appointed length of n based on a sequence length input by a user; modeling a double-stranded DNA structure for each sequence in the random unrepeated sequences and generating a corresponding double-stranded DNA three-dimensional structure file; carrying out format transformation for each generated double-stranded DNA three-dimensional structure file to be used for molecular docking; carrying out format transformation for target small molecules to enable the processed target small molecules to be used for the molecular docking; carrying out molecular docking for each target small molecule and each aptamer; and reading the score files after the molecular docking by two matrix generation functions, and respectively gType: ApplicationFiled: June 16, 2016Publication date: February 7, 2019Applicant: INSTITUTE OF ANIMAL SCIENCE OF CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Nan Zheng, Ming Li, Jiaqi Wang, Yangdong Zhang, Fang Wen, Songli Li, Shengguo Zhao
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Publication number: 20180353209Abstract: Media, kits, and methods for cultivation of mammalian embryos from fertilized oocyte stage to blastocyst stage in the presence of N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine. The use of this supplement improves the early embryonic development, in particular the quality of zygotes and the blastocyst formation. The supplemented media can be used with pigs, humans, or other mammals N-benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine may be added to known media, and used for with vitro fertilization methods.Type: ApplicationFiled: June 8, 2017Publication date: December 13, 2018Applicants: Lekarska fakulta v Plzni, Univerzita Karlova (Faculty of Medicine in Pilsen, Charles Univ), The Curators of the University of Missouri, Chonbuk National Univ, Division of Biotechnology, College of Environmental and Bioresources, Ceska zemedelska univerzita v Praze, Institute of Animal ScienceInventors: Jan Nevoral, Young-Joo Yi, Katerina Zamostna-Adamkova, Jaroslav Petr, Peter Sutovsky, Milena Kralickova
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Method for auxiliary identification of inbred line of wuzhishan miniature pig and its special primer
Patent number: 9029090Abstract: A method for auxiliary identification of WZSP inbred line and its special primers are disclosed. The method may comprise: testing whether a deoxyribose nucleotide at site 1273 from 5? end of genomic DNA comprising SEQ ID NO: 1 in a test pig is A or G, measuring whether the test pig genotype is GG, GA or AA wherein the test pig with GG genotype is a candidate for the WZSP inbred line and the test pigs with GA genotype and AA genotype are non-WZSP inbred line. The method can be applied to breed WZSP inbred line, which can be used to preliminarily screen all the pigs in test pig group, eliminate non-WZSP inbred line, find out a candidate WZSP inbred line and make a further confirmation in combination with other methods. The method and primers can also be used to judge whether a WZSP on market is counterfeiting or not.Type: GrantFiled: March 1, 2010Date of Patent: May 12, 2015Assignee: Institute of Animal Science Chinese Academy of Agricultural SciencesInventors: Shutang Feng, Kui Li, Yulian Mu, Shulin Yang -
Patent number: 8742085Abstract: A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient method for the preparation of combined-gene transferred animals.Type: GrantFiled: June 24, 2010Date of Patent: June 3, 2014Assignee: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Junhua Fan, Lijing Bai, Yulian Mu, Shulin Yang, Zhonglin Tang, Wentao Cui
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Publication number: 20130053628Abstract: A method for cultivating a transgenic animal with an increased expression amount of porcine growth hormone is provided. Additionally, a method for cultivating a transgenic embryo, which introduces a recombinant expression vector of 1) TRE-GH or 2) TET-ON into a target embryo to obtain a transgenic embryo with a higher expression amount of porcine growth hormone than a target embryo without introduction is provided. A transgenic animal can be obtained by transplanting the embryo into an animal. The experiments demonstrate that growth hormone (GH) is sharply increased in blood of transgenic pigs after adding DOX.Type: ApplicationFiled: June 24, 2010Publication date: February 28, 2013Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Xinmin Zheng, Lijing Bai, Wentao Cui, Zhonglin Tang, Yulian Mu, Shulin Yang
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METHOD FOR AUXILIARY IDENTIFICATION OF INBRED LINE OF WUZHISHAN MINIATURE PIG AND ITS SPECIAL PRIMER
Publication number: 20120315636Abstract: An auxiliary method for identification of WZSP inbred line and its special primers are provided. The method is to test if DNA at site 1273 from 5? end of genomic DNA SEQ ID NO: 1 in test pig is A or G, measure the test pig genotype is GG, GA or AA; GG or AA genotype is homozygote that DNA at site 1273 is G or A; GA genotype is their heterozygote; test pig of GG genotype is candidate for the WZSP inbred line; test pigs of GA and AA genotypes are not WZSP inbred line. The method can be applied to breed WZSP inbred line, can preliminarily screen all the pigs in test pig group, eliminate non WZSP inbred line, find out candidate WZSP inbred line, combine with other methods for further confirmation. The method can also be used to judge whether WZSP on market is counterfeiting or not.Type: ApplicationFiled: March 1, 2010Publication date: December 13, 2012Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Shutang Feng, Kui Li, Yulian Mu, Shulin Yang -
Publication number: 20120233717Abstract: A method for preparing a transgenic animal of simultaneous multiple-gene expression is provided. Additionally, a method for preparing a transgenic embryo, which introduces both phytase gene and human myxovirus resistant gene A into a target embryo, to obtain a transgenic embryo is provided. The transgenic animal of simultaneous multiple-gene expression can be achieved by transplanting the transgenic embryo into the body of a female target animal. A significant advantage of the foregoing methods, among many others, exists in that the simultaneous expression of multiple genes can be achieved in one transgenosis, which provides a convenient mean for the preparation of combined-gene transferred animals etc.Type: ApplicationFiled: June 24, 2010Publication date: September 13, 2012Applicant: Institute of Animal Science, Chinese Academy of Agricultural SciencesInventors: Kui Li, Huiming Ju, Junhua Fan, Lijing Bai, Yulian Mu, Shulin Yang, Zhonglin Tang, Wentao Cui
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Patent number: 7485290Abstract: A composition comprising Megasphaera elsdenii strain JCM1772 or strain iNP-001. The compositions may optionally contain live or processed bacterial products. A method for decreasing lactate production or increasing butyric acid production or for treating a disease associated with hyperlactate production in a non-ruminant using Megasphaera elsdenii.Type: GrantFiled: April 5, 2002Date of Patent: February 3, 2009Assignees: Kyodoken Institute for Animal Science Research & Development, Combi CorporationInventors: Kazunari Ushida, Takamitsu Tsukahara, Masaaki Okada, Hironari Koyama
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Patent number: 6451770Abstract: Disclosed and claimed is the use of a liquid jet intradermal administration apparatus that administers a composition: without a needle; and in the epidermis, dermis and/or hypodermis, such as a Pigjet apparatus, for administering bovine vaccines or immunogenic compositions, especially bovine plasmid vaccines or immunogenic compositions. Accordingly, the invention involves bovine immunogenic or vaccine compositions in such an apparatus, and methods for vaccinating bovines or for inducing an immunogenic response in bovines employing such an apparatus, as well as the apparatus containing bovine immunogenic or vaccine compositions.Type: GrantFiled: January 15, 1999Date of Patent: September 17, 2002Assignees: Merial, ID-DLO Institute of Animal Science and HealthInventors: Franciscus Antonius Maria Rijsewijk, Remco Siebren Schrijver, Johannes Theodorus Van Oirschot