Patents Assigned to Institute of Biotechnology, Academy of Military Medical Sciences, China
  • Method for preparing bacterial polysaccharide-modified recombinant fusion protein and use of the protein
    Patent number: 10844098
    Abstract: The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.
    Type: Grant
    Filed: September 1, 2015
    Date of Patent: November 24, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Chao Pan, Peng Sun, Hengliang Wang, Bo Liu, Zhehui Peng, Li Zhu, Shaohong Chang, Xin Gong, Erling Feng, Bin Wang, Ming Zeng
  • with an O-antigen having an extended carbohydrate chain and use thereof
    Patent number: 10780154
    Abstract: The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Grant
    Filed: September 7, 2015
    Date of Patent: September 22, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Hengliang Wang, Peng Sun, Bo Liu, Li Zhu, Chao Pan, Tiantian Wang, Xin Gong, Shaohong Chang, Erling Feng
  • with an O-antigen having an extended carbohydrate chain and use thereof
    Patent number: 10709777
    Abstract: The present invention discloses a Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Grant
    Filed: September 7, 2015
    Date of Patent: July 14, 2020
    Assignee: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun Wu, Peng Sun, Bo Liu, Shaohong Chang, Xin Gong, Hengliang Wang, Li Zhu, Chao Pan, Erling Feng
  • Method for Preparing Bacterial Polysaccharide-Modified Recombinant Fusion Protein and Use of the Protein
    Publication number: 20180258145
    Abstract: The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.
    Type: Application
    Filed: September 1, 2015
    Publication date: September 13, 2018
    Applicant: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun WU, Chao PAN, Peng SUN, Hengliang WANG, Bo LIU, Zhehui PENG, Li ZHU, Shaohong CHANG, Xin GONG, Erling FENG, Bin WANG, Ming ZENG
  • A Salmonella Paratyphi A with an O-Antigen Having an Extended Carbohydrate Chain and Use Thereof
    Publication number: 20180050100
    Abstract: The present invention discloses a Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cldLT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB45733-OPSSpty50973 and rEPA4573-OPSSpty50973 as prepared by using Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved.
    Type: Application
    Filed: September 7, 2015
    Publication date: February 22, 2018
    Applicant: Institute of Biotechnology, Academy of Military Medical Sciences, China
    Inventors: Jun WU, Peng SUN, Bo LIU, Shaohong CHANG, Xin GONG, Hengliang WANG, Li ZHU, Chao PAN, Erling FENG