Abstract: A method for evaluating an antiviral ability of a convalescent plasma by detecting an antibody against RBD of S protein, includes: preparing a convalescent plasma; detecting the antibody against RBD of S protein according to a principle of antigen-antibody specific binding; and evaluating the antiviral ability of the convalescent plasma according to a content of the antibody against RBD in detecting the antibody against RBD of S protein.
Type:
Application
Filed:
April 24, 2020
Publication date:
May 4, 2023
Applicant:
INSTITUTE OF BLOOD TRANSFUSION CHINESE ACADEMY OF MEDICAL SCIENCES
Abstract: The present disclosure relates to primer and probe sets for pathogen detection of infection in a transplant patient, a kit and use thereof, and belongs to the technical field of molecular biology detection. There are 23 primer and probe sets that can be used to jointly detect 23 kinds of pathogens with a high infection rate and a high lethality rate after transplantation, including an adenovirus type B; and two ends of a corresponding sequence of each probe have correspondingly a fluorophore and a quencher group, respectively. The present disclosure further provides a real-time fluorescence quantitative PCR kit for pathogen detection of infection in a transplant patient, including the primer and probe sets, a pathogen plasmid standard, a fluorescence quantitative PCR reaction solution, and sterile deionized water, which can simultaneously detect 23 pathogens infected by the transplant patient.
Type:
Application
Filed:
July 14, 2022
Publication date:
March 9, 2023
Applicant:
Institute of Blood Transfusion, Chinese Academy of Medical Sciences