Patents Assigned to Institute of Genetics
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Patent number: 11873499Abstract: The invention relates to methods for increasing nitrogen uptake, nitrogen assimilation, nitrogen use efficiency as well as yield in a plant, without affecting plant height, the method comprising increasing the expression or levels of a growth regulatory factor (GRF). Also described are genetically altered plants characterised by the above phenotype as well as methods of producing such plants.Type: GrantFiled: February 13, 2019Date of Patent: January 16, 2024Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY CHINESE ACADEMY OF SCIENCESInventors: Xiangdong Fu, Shan Li, Kun Wu, Yonghang Tian, Qian Liu
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Publication number: 20230407287Abstract: The disclosure discloses a method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution, and a use of the multisubunit SCF E3 ligase. The method for preparing a multisubunit SCF E3 ligase with a fusion protein through in vitro reconstitution disclosed by the disclsoure includes: subjecting an engineered SKP1-Cullin1-RBX1 fusion protein (referred to as eSCR) to a reaction with an F-box protein in a reaction system to obtain the multisubunit SCF E3 ligase, where the eSCR fusion protein has an amino acid sequence from position 10 to position 1062 of SEQ ID NO: 2. Experimental results show that different multisubunit SCF E3 ligases are successfully prepared with the eSCR fusion protein through in vitro reconstitution in the disclsoure; the reconstituted multisubunit E3 ligase has biological activity.Type: ApplicationFiled: May 25, 2023Publication date: December 21, 2023Applicant: Institute of Genetics and Developmental Biology, CASInventors: Jiayang LI, Bing WANG, Huihui LIU, Hong YU, Xiangbing MENG, Guifu LIU, Mingjiang CHEN, Yanhui JING
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Patent number: 11820990Abstract: Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.Type: GrantFiled: December 21, 2018Date of Patent: November 21, 2023Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Caixia Gao, Chao Li, Yuan Zong, Yanpeng Wang
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Patent number: 11767536Abstract: The present invention discloses a method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution, and also relates to a method capable of generating a site-directed nucleotide substitution and a fragment substitution. The method for obtaining a glyphosate-resistant plant provided by the present invention comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I), and substituting proline (P) at position 12 with serine (S) to obtain a plant, i.e., a glyphosate-resistant plant. The method provided by the present invention is of great significance in breeding new herbicide-resistant plant varieties.Type: GrantFiled: August 15, 2016Date of Patent: September 26, 2023Assignee: Institute of Genetics and Developmental Biology, Chinese Academy of SciencesInventors: Caixia Gao, Jun Li
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Patent number: 11739313Abstract: A method of modifying an upstream open reading frame (uORF) by genome editing technique to regulate gene expression.Type: GrantFiled: October 19, 2018Date of Patent: August 29, 2023Assignees: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES, TIANJIN GENOVO BIOTECHNOLOGY CO., LTD.Inventors: Caixia Gao, Huawei Zhang, Yidong Ran
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Patent number: 11739322Abstract: Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.Type: GrantFiled: January 31, 2019Date of Patent: August 29, 2023Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Caixia Gao, Huawei Zhang, Shuai Jin
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Publication number: 20230257761Abstract: The present invention relates to the field of plant genetic engineering. In particular, the present invention relates to acetolactate synthase (ALS) mutants, protoporphyrinogen oxidase (PPO) mutants, acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants capable of conferring herbicide resistance in plants, especially in wheat and/or rice plants, and methods for production herbicide-resistant plants, especially wheat and/or rice plants comprising said acetolactate synthase (ALS) mutants and/or protoporphyrinogen oxidase (PPO) mutants and/or acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants.Type: ApplicationFiled: September 11, 2020Publication date: August 17, 2023Applicants: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES, TIANJIN GENOVO BIOTECHNOLOGY CO., LTD.Inventors: Caixia GAO, Yuhang CHEN, Yidong RAN, Rui ZHANG, Chao LI, Sha CHEN, Kang ZHANG, Hu XU
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Patent number: 11725214Abstract: The invention relates to methods for increasing plant yield, and in particular grain yield by reducing or abolishing the expression and/or activity of OTUB1 in a plant. Also described are genetically altered plants characterised by the above phenotype and methods of producing such plants.Type: GrantFiled: May 24, 2018Date of Patent: August 15, 2023Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY CHINESE ACADEMY OF SCIENCESInventors: Xiangdong Fu, Shuansuo Wang, Kun Wu, Qian Liu, Qian Qian, Ke Huang, Penggen Duan, Baolan Zhang, Yunhai Li
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Publication number: 20230227835Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.Type: ApplicationFiled: August 19, 2022Publication date: July 20, 2023Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Caixia GAO, Yuan ZONG
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Publication number: 20220411810Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.Type: ApplicationFiled: August 19, 2022Publication date: December 29, 2022Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Caixia GAO, Yi ZHANG, Zhongyi WU, Kang ZHANG
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Patent number: 11519001Abstract: This invention relates to a plant E3 ubiquitin ligase (termed DA2) which acts synergistically with DA1 to control seed and organ size. Methods of increasing plant yield are provided that comprise reducing the expression or activity of DA2 in a plant that is deficient in DA1 expression or activity. Plants with increased yield and methods of producing such plants are also provided.Type: GrantFiled: April 17, 2019Date of Patent: December 6, 2022Assignees: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY CHINESE ACADEMY OF SCIENCES, PLANT BIOSCIENCE LIMITEDInventors: Yunhai Li, Tian Xia, Na Li, Jack Dumenil, Michael Bevan
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Patent number: 11447785Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.Type: GrantFiled: November 14, 2017Date of Patent: September 20, 2022Assignee: Institute of Genetics and Developmental Biology, Chinese Academv of SciencesInventors: Caixia Gao, Yuan Zong
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Publication number: 20220267788Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.Type: ApplicationFiled: April 29, 2022Publication date: August 25, 2022Applicants: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES, CHINA AGRICULTURAL UNIVERSITYInventors: Caixia GAO, Linjian JIANG
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Patent number: 11421241Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.Type: GrantFiled: January 27, 2016Date of Patent: August 23, 2022Assignee: Institute of Genetics and Developmental Biology, Chinese Academy of SciencesInventors: Caixia Gao, Yi Zhang, Zhongyi Wu, Kang Zhang
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Publication number: 20220251580Abstract: Provided is a gene editing system for editing a target sequence in the genome of a cell, comprising a CRISPR nuclease, a cytosine deaminase, an AP lyase, a guide RNA and optionally an uracil-DNA glycosylase. Also provided are a method of producing a genetically modified cell, and a kit comprising the gene editing system.Type: ApplicationFiled: May 7, 2020Publication date: August 11, 2022Applicant: Institute of Genetics and Developmental Biology, Chinese Academy of SciencesInventors: Caixia Gao, Huawei Zhang, Shengxing Wang
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Patent number: 11345924Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.Type: GrantFiled: May 11, 2018Date of Patent: May 31, 2022Assignees: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCES, CHINA AGRICULTURAL UNIVERSITYInventors: Caixia Gao, Linjian Jiang
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Patent number: 11213613Abstract: Provided is a three dimensional tissue scaffold comprising a stem cell attracting element associated with a matrix, and fusion protein of stem cell attracting factor and collagen-binding domain, and methods of uses thereof.Type: GrantFiled: September 30, 2015Date of Patent: January 4, 2022Assignee: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Jianwu Dai, Jie Sun, Bing Chen, Zhifeng Xiao
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Publication number: 20210403901Abstract: The present invention relates to novel methods for discovering traits and generating cellular systems having improved phenotypes. In particular, the present invention provides methods for the development of plants having agronomically optimized phenotypes by using targeted mutagenesis with few or no off-target effects. Targeted mutagenesis is achieved by the introduction of a base editor complex or of a STEME complex comprising an array of guide RNAs targeting a nucleic acid sequence of interest. The present invention also relates to cellular systems obtained by the methods described herein and to the use of a base editor complex or the STEME complex comprising an array of guide RNAs for generating a cellular system having an agronomically important phenotype and for identification of an agronomically important phenotype.Type: ApplicationFiled: November 4, 2019Publication date: December 30, 2021Applicant: Institute of Genetics and Developmental Biology, Chinese Academy of SciencesInventors: Yu Mei, Aaron Hummel, Zarir Vaghchhipawala, Caixia Gao, Chao Li, Rui Zhang
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Publication number: 20210230616Abstract: The present invention relates to methods for targeted editing in a plant, a plant cell or material, which is combined with the parallel introduction of a phenotypically selectable trait. Furthermore, methods are provided not comprising a step of introducing a transgenic selection marker sequence.Type: ApplicationFiled: May 7, 2018Publication date: July 29, 2021Applicant: INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Caixia GAO, Rui ZHANG, Jinxing LIU, Aaron HUMMEL, Zarir VAGHCHHIPAWALA, Mathias LABS
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Publication number: 20210207290Abstract: A method for isolating exosomes from a biological sample may include synthesizing a first nanofibrous substrate by coaxial electrospinning of a core polymer solution and a shell polymer solution. The shell polymer solution may include gelatin. A method for isolating exosomes may further include obtaining a second nanofibrous substrate by immobilizing an antibody, such as CD63, CD9, and CD81 to the first nanofibrous substrate, capturing the exosomes from the biological sample by incubating the biological sample with the second nanofibrous substrate, and releasing the captured exosomes from the second nanofibrous substrate by incubating the second nanofibrous substrate with water at a temperature of 37° C.Type: ApplicationFiled: March 19, 2021Publication date: July 8, 2021Applicants: National institute of genetic engineering and biotechnologyInventor: Matin Mahmoudifard