Abstract: The present disclosure discloses a method for inhibiting formation of a biofilm of bacteria. Specifically, the method may include treating the bacteria with an effective amount of a coumarin-chalcone compound. While inhibiting the formation of the biofilm of the bacteria, the effective amount of the coumarin-chalcone compound may reduce virulences of the bacteria and enhance a susceptibility of the bacteria to an antibiotic when applied in combination with the antibiotic. The present disclosure further discloses a composition including an effective amount of the coumarin-chalcone compound. The composition may be used to inhibit the formation of the biofilm of bacteria. The composition may also include an antibiotic, a minimal inhibitory concentration and a minimal biofilm eliminate concentration of which are reduced when the composition is used.

Abstract: Provided in the present invention are a humanized monoclonal antibody for the 2019 novel coronavirus (2019-nCOV) and the use thereof. The antibody is capable of specifically binding with a receptor binding domain (RBD) of 2019-nCOV and blocking the binding between the RBD of 2019-nCOV and ACE2, and furthermore inhibiting the infection caused by 2019-nCOV.

Abstract: The embodiments of the present disclosure relate to antigens of ?-coronaviruses, preparation methods and uses thereof. The amino acid sequence of the antigen of the ?-coronavirus includes an amino acid sequence arranged in a (A-B)-(A-B) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B) pattern or an amino acid sequence arranged in a (A-B)-(A-B?) pattern or an amino acid sequence arranged in a (A-B)-C-(A-B?) pattern. The antigen of the ?-coronavirus has a single-chain dimer structure. A single-chain dirtier expressed according to examples of the present disclosure is stable in content and has excellent immunogenicity as an antigen of a ?-coronavirus, and a vaccine prepared by using the single-chain dimer as an antigen of a ?-coronavirus can elicit high-titer neutralizing antibodies in mice.

Abstract: The present invention discloses a method for modifying an amino acid attenuator, a class of amino acid attenuator mutants, engineered bacteria created on the basis of the amino acid attenuator mutants, and use of the engineered bacteria. The present invention protects a method for relieving the attenuation regulation of an amino acid operon gene, which is modification of the amino acid operon gene by: removing a gene coding for a leader peptide and an anterior reverse complementary palindromic sequence in the terminator stem-loop structure, and maintaining a posterior reverse complementary palindromic sequence in the terminator. The amino acid operon particularly can be histidine operon, tryptophan operon, phenylalanine operon, alanine operon, threonine operon and etc. The present invention can be used for the production of amino acids and derivatives thereof in fermentation by bacteria, providing a novel method for improving the production of amino acids in fermentation.

Abstract: Compositions for and methods of treating irritable bowel disease are provided. The compositions and methods employ MG53, which can be in the form of recombinant human MG53. The MG53 can be administered in a form to avoid or bypass gastric digestion or degradation. The composition can include a live MG53-expressing microbe, such as a probiotic composition that expresses MG53 in the gastrointestinal tract of a subject. An enteric release composition comprising MG53 is also provided.

Abstract: The present disclosure discloses a method for inhibiting formation of a biofilm of bacteria. Specifically, the method may include treating the bacteria with an effective amount of a coumarin-chalcone compound. While inhibiting the formation of the biofilm of the bacteria, the effective amount of the coumarin-chalcone compound may reduce virulences of the bacteria and enhance a susceptibility of the bacteria to an antibiotic when applied in combination with the antibiotic. The present disclosure further discloses a composition including an effective amount of the coumarin-chalcone compound. The composition may be used to inhibit the formation of the biofilm of bacteria. The composition may also include an antibiotic, a minimal inhibitory concentration and a minimal biofilm eliminate concentration of which are reduced when the composition is used.

Abstract: A nanoparticle comprises water-insoluble polymer matrix and an indicator constituent(s), wherein the indicator constituent(s) is released from the nanoparticle only when the polymer matrix is degraded or broken, and then an indicative effect is triggered or enhanced. A nanoparticle-based method for screening a bioactive substance and a microfluidic-based screening system have also been disclosed.

Abstract: Provided is a method for construction of recombinant bacteria for producing 3-hydroxypropionic acid. The method includes: knocking out fadR, fabF and fabH genes of recipient bacteria, introducing acc genes or gene clusters, alKL and Mcr genes, and enhancing the expression of fadL, fadD, sthA genes and atoSC gene clusters in the recipient bacteria. Also provided is a method for producing 3-hydroxypropionic acid by using the recombinant bacteria.

Abstract: The present invention relates to a magnetic holder for immunoelectron microscopy grids. The holder comprises a frame, a magnet and a hydrophobic layer. The device can use a magnetic force to simultaneously attach the outer rings of nickel grids to the frame, so that a batch operation (such as rinsing, immunolabeling and dyeing) of the nickel grids can be realized. In addition, due to the hydrophobic effect of the hydrophobic layer, the holder can reduce the amount of the liquid carried by the nickel grids in the process of continuously transferring the nickel grids between different types of liquids to almost zero. Compared with the prior art, the magnetic holder effectively reduces the probability of cross-contamination between reagents.

Abstract: The present invention relates to a dual-arm clamping type holder for transmission electron microscopy grids and preparation method thereof. The preparation method comprises: firstly manufacturing a frame with two adjacent arms located on a same plane and have a clamping structure by a hard material; then putting the frame in a molten adhering liquid so that the frame is dipped with the adhering liquid; and finally, taking out the frame dipped with the adhering liquid, and waiting for the adhering liquid to solidify into adhering layers along the arms. The dual-arm clamping type holder manufactured by the method of the present invention comprises a frame and adhering layers; and the adhering layers adhere to the inner sides of the clamping structure between the two adjacent arms of the frame.

Abstract: The present invention relates to a magnetic holder for immunoelectron microscopy grids. The holder comprises a frame, a magnet and a hydrophobic layer. The device can use a magnetic force to simultaneously attach the outer rings of nickel grids to the frame, so that a batch operation (such as rinsing, immunolabeling and dyeing) of the nickel grids can be realized. In addition, due to the hydrophobic effect of the hydrophobic layer, the holder can reduce the amount of the liquid carried by the nickel grids in the process of continuously transferring the nickel grids between different types of liquids to almost zero. Compared with the prior art, the magnetic holder effectively reduces the probability of cross-contamination between reagents.

Abstract: The present invention relates to a dual-arm clamping type holder for transmission electron microscopy grids and preparation method thereof. The preparation method comprises: firstly manufacturing a frame with two adjacent arms located on a same plane and have a clamping structure by a hard material; then putting the frame in a molten adhering liquid so that the frame is dipped with the adhering liquid; and finally, taking out the frame dipped with the adhering liquid, and waiting for the adhering liquid to solidify into adhering layers along the arms. The dual-arm clamping type holder manufactured by the method of the present invention comprises a frame and adhering layers; and the adhering layers adhere to the inner sides of the clamping structure between the two adjacent arms of the frame.

Abstract: Provided are key amino acid sites regulating the nuclear export of a nucleoprotein in influenza A and B viruses and the use thereof as anti-influenza virus drug targets. Also provided is a pharmaceutical composition for treating the influenza A or B virus infection, comprising a compound that regulates the nuclear export of a nucleoprotein in the influenza A or B virus.

Abstract: A recombinant virus is obtained by mutating a codon that encodes a tyrosine residue at position 385 of NP protein in the genome of influenza A virus to a codon of phenylalanine residue. The virus WSN-Y385F is a temperature-sensitive virus that can normally replicate and survive at 37° C., and cannot normally replicate and cannot survive at 33° C. Phosphorylation of a NP protein of influenza A virus can be inhibited by mutating an amino acid residue at position 385 from N terminal of the NP protein of influenza A virus, from a tyrosine to a phenylalanine. The recombinant virus can be used in analyzing mechanisms of infection by influenza virus, and in connection with methods of prevention and treatment of infection by influenza virus.

Abstract: The present disclosure provides an inhibitor for inhibiting or disrupting biofilm and use thereof. Specifically, the present disclosure an application of a PsIG protein and a coding sequence thereof in inhibiting or disrupting a biofilm of a microorganism. The present disclosure also provides a composition and a preparation containing the PsIG protein to inhibit or disrupt a biofilm of a microorganism. The experiment shows that PsIG can effectively inhibit or disrupt a biofilm of Pseudomonas species, suggesting the great potential of PsIG in clinical and environmental fields.

Abstract: Aromatic farnesyl compound and application of a pharmaceutical salt thereof as an inhibitor of ?-glucosidase, dipeptidyl peptidase-4, aldose reductase, protein tyrosine phosphatase-1B or HMG-CoA reductase, or use thereof in preparing medicine and functional healthcare products having a liver protective function and/or for treating and/or preventing type II diabetes, diabetic retinopathy, diabetic foot disease, and hyperlipidemia.

Abstract: A droplet generating apparatus, system, and method are disclosed. Based on a relative vibration between a micro-pipe and a container containing a second liquid, a first liquid flowing out from an outlet end of the micro-pipe can be detached from the micro-pipe by a fluid shear force of the second liquid to form droplets in the second liquid. Multitudinous quantitative and uniform droplets can be generated precisely and accurately in the present disclosure.

Abstract: The invention provides a polypeptide capable of using 4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP) as a substrate to produce isoprene, a nucleic acid encoding the polypeptide, and a vector and a cell comprising the nucleic acid. In addition, the invention also provides a method for producing isoprene using the polypeptide, and a method for preparing the polypeptide.

Abstract: Methods for removing, reducing, dispersing, disrupting or eradicating biofilms present on a surface may include contacting the biofilm with a composition including an agent that reduces the potency of an aminopeptidase in the biofilm. The agent may reduce expression, secretion or extracellular activity of the aminopeptidase in the biofilm. The biofilm may be formed by P. aeruginosa or P. stutzeri. The composition may enhance the sensitivity of P. aeruginosa to ciprofloxacin. Methods of determining the number of bacterial population in biofilm or a culture containing bacterial aggregate may include dispersing the bacterial aggregate or biofilm by using a composition comprising exogenous PslG. The number of bacteria may be determined based on the dispersed bacteria.

Abstract: The present disclosure provides an inhibitor for inhibiting or disrupting biofilm and use thereof. Specifically, the present disclosure an application of a PsIG protein and a coding sequence thereof in inhibiting or disrupting a biofilm of a microorganism. The present disclosure also provides a composition and a preparation containing the PsIG protein to inhibit or disrupt a biofilm of a microorganism. The experiment shows that PsIG can effectively inhibit or disrupt a biofilm of Pseudomonas species, suggesting the great potential of PsIG in clinical and environmental fields.