Abstract: An exosome active formulation for inhibiting endothelial cell migration, and a preparation method and use thereof include the following steps: isolating primary umbilical vein endothelial cells and performing cell culture and passage; adding anisodamine to a culture medium of the subcultured endothelial cells to pretreat the endothelial cells, and then replacing the culture medium with a new endothelial cell culture medium and adding TNF-? to continue the culture of the endothelial cells; extracting exosomes from the TNF-? containing endothelial cell culture medium obtained after the endothelial cells are cultivated; and identifying the exosomes. The exosome active formulation for inhibiting endothelial cell migration prepared by the preparation method of this disclosure and its use in the antitumor drugs.
Type:
Grant
Filed:
April 29, 2019
Date of Patent:
April 6, 2021
Assignee:
INSTITUTE OF MICROCIRCULATION (OF THE CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNION MEDICAL COLLEGE)
Abstract: An exosome active formulation for promoting endothelial cell angiogenesis, and a preparation method and use thereof include the following steps: isolating primary umbilical vein endothelial cells and performing cell culture and passage; adding anisodamine to a culture medium of the subcultured endothelial cells to treat the endothelial cells, and then replacing the culture medium with a new endothelial cell culture medium to continue the culture of the endothelial cells; extracting exosomes from the endothelial cell culture medium obtained after the endothelial cells are cultivated; and identifying the exosomes. The exosome active formulation for promoting endothelial cell angiogenesis prepared by the preparation method and its use in the manufacture of a medicament for treating microvascular injury, microcirculation dysfunction and cardio-cerebral infarction.
Type:
Grant
Filed:
April 29, 2019
Date of Patent:
March 30, 2021
Assignee:
INSTITUTE OF MICROCIRCULATION (OF THE CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNION MEDICAL COLLEGE)