Patents Assigned to INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES
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Patent number: 11859245Abstract: A primer set for detecting telomerase activity, the primer set including a first primer set or a second primer set. The first primer set includes: an upstream primer selected from MTS; and a downstream primer selected from the group consisting of ACX-M4, Beacon ACX62-2C, and Beacon ACX62-10. The second primer set includes: an upstream primer selected from STS or CTS; and a downstream primer selected from the group consisting of ACX, CXT, ACX-M4, Beacon ACX62-2C, or Beacon ACX62-10. The sequences of the primers ACX, CXT, ACX-M4, Beacon ACX62-2C, Beacon ACX62-10, STS, CTS and MTS are shown as SEQ ID NOs: 1 to 8, respectively.Type: GrantFiled: July 27, 2020Date of Patent: January 2, 2024Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Zheng Tan, Kewei Zheng, Yuhua Hao
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Publication number: 20230365927Abstract: Provided are a composition for inducing cell transdifferentiation and use thereof in inducing the transdifferentiation of non-neuronal cells into neurons. The composition comprises a myosin inhibitor, and an isoxazole compound and/or a derivative thereof. Further provided is a method for inducing the transdifferentiation of non-neuronal cells into neurons, comprising: culturing the non-neuronal cells in an induction culture solution comprising a myosin inhibitor, and then culturing them in a mature culture solution comprising a myosin inhibitor, and an isoxazole compound and/or a derivative thereof, so as to obtain mature neurons. Also provided is a method for transdifferentiating non-neuronal cells within a subject into neurons, comprising administering to the subject an effective amount of a myosin inhibitor, and an isoxazole compound and/or a derivative thereof.Type: ApplicationFiled: October 23, 2020Publication date: November 16, 2023Applicants: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES, BEIJING INSTITUTE FOR STEM CELL AND REGENERATIVE MEDICINEInventors: Wei LI, Qi ZHOU, Zhengquan HE
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Patent number: 11767525Abstract: Provided are a genome editing system CRISPR-C2c1 for site-specific modification of target sequences in a cell genome and the use thereof, wherein the system comprises a C2c1 protein or variants thereof and a guide RNA. Also provided are a method for site-specific modification of target sequences in a cell genome using the genome editing system CRISPR-C2c1, and a pharmaceutical composition comprising the genome editing system CRISPR-C2c1.Type: GrantFiled: December 27, 2017Date of Patent: September 26, 2023Assignee: Institute of Zoology, Chinese Academy of SciencesInventors: Wei Li, Qi Zhou, Fei Teng
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Patent number: 11702643Abstract: The invention relates to the field of genetic engineering. In particular, the present invention relates to a novel eukaryotic genome editing system and method. More specifically, the present invention relates to a CRISPR-Cpf1 system capable of efficiently editing a genome of a eukaryotic cell and the use thereof.Type: GrantFiled: April 10, 2018Date of Patent: July 18, 2023Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Wei Li, Qi Zhou, Fei Teng
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Patent number: 11622964Abstract: Disclosed are a method for destroying cellular mechanical homeostasis and the use thereof, especially disclosed is a method for destroying cellular mechanical homeostasis by using a Myosin inhibitor thereby leading to cell softening and reduction of fibrosis in tissues and organs. Also disclosed are a method for promoting regeneration and repair of tissues and organs and the use thereof. The Myosin inhibitor can destroy the homeostasis of the cellular mechanical stress system, reduce the rigidity of tissues and organs in a pathological state, and stimulate stress and regeneration reactions similar to those in the regeneration processes in lower organisms; and can use the characteristics to greatly inhibit fibrosis during the organ damage and to promote the regeneration and repair of tissues and organs, and meanwhile can use stress response to greatly improve the ability of cellular genetic repair. The Myosin inhibitor is preferably (?)-Blebbistatin, or a derivative thereof (?)-Blebbistatin O-Benzoate.Type: GrantFiled: January 29, 2019Date of Patent: April 11, 2023Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Qi Zhou, Wei Li, Zhengquan He, Liu Wang
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Patent number: 11547097Abstract: A hybridization method of Sinocyclocheilus grahami and carp, which comprises parent fish cultivation, artificial insemination, fish egg incubating and fry feeding, strictly controls environmental parameters and feed types of parent fish cultivation, simulates good living conditions for S. grahami and carp, is beneficial for wild S. grahami and carp to adapt to the artificial breeding environment quickly, realizes natural spermatogenesis and oviposition in artificial environment without using the oxytocin, and provides sperm and eggs both in quality and quantity for artificial hybridization. At the same time, the key parameters during incubation (including incubating water level, water temperature, water flow velocity, pH, dissolved oxygen and sterilization of fertilized eggs, etc.) are also defined to provide suitable environmental conditions for the successful incubation of fertilized eggs.Type: GrantFiled: March 17, 2021Date of Patent: January 10, 2023Assignee: KUNMING INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yuanwei Zhang, Xiao'ai Wang, Xiaofu Pan, Junxing Yang
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Patent number: 11447470Abstract: Disclosed is a pyrimidinone-containing compound represented by formula I, a preparation method thereof, a pharmaceutical composition, and an application thereof. The pyrimidinone-containing compound of the present disclosure can be used as HIV-1 inhibitor and can be also used in the treatment of human immunodeficiency virus infection.Type: GrantFiled: March 4, 2019Date of Patent: September 20, 2022Assignees: Yunnan University, Kunming Institute of Zoology, Chinese Academy of SciencesInventors: Yanping He, Hongbin Zhang, Yongtang Zheng, Yumeng Wu, Chengrun Tang, Ruomei Rui, Liumeng Yang, Jiangyuan Wang
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Patent number: 11419320Abstract: Disclosed is a cold-resistant and lean-type transgenic pig and a preparation method therefor, which relate to the field of genetic engineering. By transferring a mouse uncoupling protein 1 gene into the genome of a pig, a transgenic pig is obtained which can not only resist the cold but also have an increased lean meat rate by reducing fat deposition. Simultaneous improvement of two important production traits of pigs through the site-directed single gene manipulation not only lays a foundation for the application and basic research of genetic editing for big animals, but also provides with breading researchers a new way of thinking for improving traits of livestock.Type: GrantFiled: December 29, 2017Date of Patent: August 23, 2022Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Jianguo Zhao, Qiantao Zheng, Guosong Qin, Jing Yao, Chunwei Cao
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Patent number: 11420959Abstract: Disclosed is a DACOs-type NNRTIs amino acid ester derivative, a preparation method thereof, a pharmaceutical composition, and an application thereof. The structure of the DACOs-type NNRTIs amino acid ester derivative is represented by formula (I). The DACOs-type NNRTIs amino acid ester derivative represented by formula (I) can be used as HIV-1 inhibitors and for the preparation of a drug for treating and/or preventing immunodeficiency viruses.Type: GrantFiled: March 4, 2019Date of Patent: August 23, 2022Assignees: Yunnan University, Kunming Institute of Zoology, Chinese Academy of SciencesInventors: Yanping He, Hongbin Zhang, Yongtang Zheng, Yufang Zhang, Chengrun Tang, Wei Ding, Liumeng Yang, Yiming Li
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Publication number: 20210381038Abstract: A method for detecting the presence and/or amount of a target nucleic acid molecule in a biological sample and a related kit. The method comprises: (a) contacting the biological sample with: i) Cas12b protein, ii) a gRNA targeting a target sequence of the target nucleic acid molecule, and iii) a single-stranded DNA reporter molecule generating a detectable signal after being cleaved to form a reaction mixture; and (b) detecting the presence and/or level of the detectable signal generated in the reaction mixture.Type: ApplicationFiled: November 30, 2018Publication date: December 9, 2021Applicant: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Wei Li, Qi Zhou, Fei Teng
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Publication number: 20210230550Abstract: The present invention relates to a cell induction method, in particular to a method for inducing differentiation of fibroblasts into adipocytes, comprising the following steps: Cultivating the fibroblasts in a culture medium, adding a Myosin inhibitor and BMP4 to the culture medium, and continuously cultivating same until adipocytes are obtained. The present invention further relates to a method for inducing the conversion of fibroblasts into immortalized cells and use thereof. The method comprises the following steps: cultivating the fibroblasts in a culture medium, adding Myosin inhibitor to the culture medium, and continuously cultivating same until immortalized cells are obtained. The present invention further relates to a method for inducing in vitro amplification of hepatocytes.Type: ApplicationFiled: January 29, 2019Publication date: July 29, 2021Applicant: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Qi ZHOU, Wei Ll, Zhengquan HE, Liu WANG
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Publication number: 20210095271Abstract: Provided are a genome editing system CRISPR-C2c1 for site-specific modification of target sequences in a cell genome and the use thereof, wherein the system comprises a C2c1 protein or variants thereof and a guide RNA. Also provided are a method for site-specific modification of target sequences in a cell genome using the genome editing system CRISPR-C2c1, and a pharmaceutical composition comprising the genome editing system CRIPSR-C2c1.Type: ApplicationFiled: December 27, 2017Publication date: April 1, 2021Applicant: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Wei Li, Qi Zhou, Fei Teng
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Publication number: 20210069177Abstract: Disclosed are a method for destroying cellular mechanical homeostasis and the use thereof, especially disclosed is a method for destroying cellular mechanical homeostasis by using a Myosin inhibitor thereby leading to cell softening and reduction of fibrosis in tissues and organs. Also disclosed are a method for promoting regeneration and repair of tissues and organs and the use thereof. The Myosin inhibitor can destroy the homeostasis of the cellular mechanical stress system, reduce the rigidity of tissues and organs in a pathological state, and stimulate stress and regeneration reactions similar to those in the regeneration processes in lower organisms; and can use the characteristics to greatly inhibit fibrosis during the organ damage and to promote the regeneration and repair of tissues and organs, and meanwhile can use stress response to greatly improve the ability of cellular genetic repair. The Myosin inhibitor is preferably (?)-Blebbistatin, or a derivative thereof (?)-Blebbistatin O-Benzoate.Type: ApplicationFiled: January 29, 2019Publication date: March 11, 2021Applicant: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Qi ZHOU, Wei Ll, Zhengquan HE, Liu WANG
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Publication number: 20210046154Abstract: The present invention relates to a method of treating, mitigating, minimizing, or preventing HIV-1/AIDS by administering a CD24 protein to a subject in need thereof. Also provided herein is use of a CD24 protein in the manufacture of a medicament for treating HIV-1/AIDS. Further, provided is a pharmaceutical composition comprising a pharmaceutically acceptable amount of a CD24 protein.Type: ApplicationFiled: March 5, 2019Publication date: February 18, 2021Applicants: ONCOIMMUNE, INC, UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, INSTITUTE OF BIOPHYSICS, CHINESE ACADEMY OF SCIENCES, KUNMING INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Yang Liu, Pan Zhang, Lishan Su, Yong-Tang Zheng, Liguo Zhang
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Publication number: 20200253175Abstract: Disclosed is a cold-resistant and lean-type transgenic pig and a preparation method therefor, which relate to the field of genetic engineering. By transferring a mouse uncoupling protein 1 gene into the genome of a pig, a transgenic pig is obtained which can not only resist the cold but also have an increased lean meat rate by reducing fat deposition. Simultaneous improvement of two important production traits of pigs through the site-directed single gene manipulation not only lays a foundation for the application and basic research of genetic editing for big animals, but also provides with breading researchers a new way of thinking for improving traits of livestock.Type: ApplicationFiled: December 29, 2017Publication date: August 13, 2020Applicant: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Jianguo ZHAO, Qiantao ZHENG, Guosong QIN, Jing YAO, Chunwei CAO
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Publication number: 20190183851Abstract: A use of 3,4,7-trihydroxyisoflavone or 3-methoxydaidzein is provided for implementation in preparation of a drug for inhibiting platelet aggregation. The 3,4,7-trihydroxyisoflavone or 3-methoxydaidzein has a significant inhibition effect on platelet aggregation, and bleeding test conducted with these embodiments show that all of the mice administrated with 3,4,7-trihydroxyisoflavone and 3-methoxydaidzein did not show any significant hemorrhagic activity, while the control group, i.e., the group administrated with the same concentration of clopidogrel showed very significant hemorrhagic activity. Thus, the use of such a drug of the present invention is useful in inhibiting platelet aggregation, and the use of such a drug can reduce the risk of bleeding, is safe for use, and expands the clinical and medical applications thereof.Type: ApplicationFiled: May 3, 2017Publication date: June 20, 2019Applicant: Kunming Institute of Zoology, Chinese Academy of SciencesInventors: Ren Lai, Chuanbin Shen, Qiumin Lv, Ming Liu
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Patent number: 10155808Abstract: A monoclonal antibody against human PrPc protein capable of reducing the expression level of transcription factor Twist1 in a targeting mode and inducing macrophage and NK cells to target to rectal cancer tumor cells. The combined drug therapy with the antibody and cetuximab also exhibits inhibitory effect on tumor better than administration of the antibody or cetuximab alone.Type: GrantFiled: November 14, 2016Date of Patent: December 18, 2018Assignee: Institute of Zoology, Chinese Academy of SciencesInventors: Quan Chen, Baowei Li, Haiying Hang, Lei Du, Jun Wang, Xiaohui Wang
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Patent number: 10112918Abstract: The invention relates to a derivative of 15-oxospiramilacton, particularly to a compound of Formula I or II, or an isomer, a solvate or a pharmaceutically acceptable salt thereof. The invention also relates to a pharmaceutical composition comprising the compound as pharmaceutically active ingredient, a method for preparing the same, and use thereof in manufacture of an anti-tumor agent. The derivative of 15-oxospiramilactone of the invention have an activity against multiple tumor cell lines, and the anti-tumor activity is positively correlated to an activity inhibiting the Wnt signaling pathway.Type: GrantFiled: April 21, 2014Date of Patent: October 30, 2018Assignees: Kunming Institute of Botany, The Chinese Academy of Sciences, The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Science, Shanghai Institutes of Biological Sciences, CAS, Institute of Zoology, Chinese Academy of SciencesInventors: Xiaojiang Hao, Chen Yan, Haiyang Liu, Lin Li, Xiaoli He, Quan Chen
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Patent number: 9944901Abstract: Provided is a protein coded by a gene related to cell totipotency and a transcriptional activation domain of a mammalian YAP protein or a fusion protein of a segment with a transcriptional control activity, a coding nucleotide sequence, an expression vector and a composition thereof, as well as a method for inducing the pluripotent stem cells by using the fusion protein.Type: GrantFiled: June 17, 2014Date of Patent: April 17, 2018Assignees: Institute of Zoology, Chinese Academy of Sciences, Emory University School of MedicineInventors: Dahua Chen, Peng Jin, Qinmiao Sun, Weiqi Tan
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Patent number: 9617318Abstract: A cDNA, including the nucleotide sequence represented by SEQ ID NO: 2. A cDNA, including a nucleotide sequence encoding a complete protein, a protein fragment, a protein analog, or a protein derivative each including the amino acid sequence represented by SEQ ID NO: 1. A cDNA, including the nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 1.Type: GrantFiled: November 15, 2016Date of Patent: April 11, 2017Assignee: INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCESInventors: Zheng Tan, Yong Zhao, Feng Wang, Yuhua Hao