Abstract: A method for detecting and quantifying viral or subviral pathogens in the test sample in which antibodies against a component of the viral or subviral pathogen is immobilized on a substrate. The test sample is placed in contact with the immobilized antibody and then a DNA primer complementary to a fragment or the total genome of the viral or subviral pathogen is permitted to hybridize to the RNA or DNA of the immobilized viral or subviral pathogen. The DNA is then amplified and the amplification products are detected and quantified.
Type:
Grant
Filed:
February 14, 1995
Date of Patent:
February 3, 1998
Assignee:
Instituto Nacional de Investigacion y Techologia Agraria y Alimentaria
Inventors:
Gustavo Nuno Bardosa Nolasco, Carmen De Blas Beorlegui, Maria Jose Borja Tome, Fernando Pons Ascaso, Vincente Torres Pascual