Patents Assigned to IntegenX, Inc.
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Patent number: 8894946Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.Type: GrantFiled: October 19, 2012Date of Patent: November 25, 2014Assignee: IntegenX Inc.Inventors: William D. Nielsen, Richard J. Belcinski, Gregory Bogdan, David Eberhart, Omar El-Sissi, Stevan B. Jovanovich, Michael Recknor, Ezra Van Gelder, David W. Wyrick
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Patent number: 8763642Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.Type: GrantFiled: August 20, 2011Date of Patent: July 1, 2014Assignee: IntegenX Inc.Inventor: Mattias Vangbo
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Publication number: 20140170645Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: ApplicationFiled: August 15, 2013Publication date: June 19, 2014Applicant: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
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Patent number: 8748165Abstract: This invention provides a method for generating short tandem repeat (STR) profiles on each of a plurality of samples comprising, for each sample: a) isolating DNA from the sample; b) amplifying STR markers in the isolated DNA and c) analyzing the amplification product by electrophoresis.Type: GrantFiled: August 21, 2012Date of Patent: June 10, 2014Assignee: IntegenX Inc.Inventors: Mattias Vangbo, William D. Nielsen, Iuliu I. Blaga, Michael Van Nguyen, Steven B. Jovanovich
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Patent number: 8672532Abstract: This invention provides microfluidic devices that comprise a fluidics layer having microfluidic channels and one or more regulating layers that regulate the movement of fluid in the channels. The microfluidic devices can be used to mix one or more fluids. At least a portion of the fluidics layer can be isolated from the regulating layer, for example in the form of a shelf. Such isolated portions can be used as areas in which the temperature of liquids is controlled. Also provided are instruments including thermal control devices into which the microfluidic device is engaged so that the thermal control device controls temperature in the isolated portion, and a movable magnetic assembly including magnets with shields so that a focused magnetic field can be applied to or withdrawn from the isolated portion or any other portion of the microfluidic device. Also provided are methods of mixing fluids.Type: GrantFiled: December 18, 2009Date of Patent: March 18, 2014Assignee: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, Michael Van Nguyen
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Publication number: 20140065628Abstract: The disclosure provides methods and devices for separating and detecting nucleic acid fragments labeled with a plurality of spectrally resolvable dyes using a single light source or multiple light sources. Use of a greater number of light sources increases the number of spectrally resolvable dyes that can be interrogated. Labeling fragments with a greater number of spectrally resolvable dyes permits more overlapping of fragments with differentiation of the fragments, and thus separation can be conducted on a smaller range of fragment sizes/lengths. To improve the detection sensitivity of a detection system employing multiple light sources, light emitted by the light sources can be spatially separated from one another and/or the intensity of each of the light sources can be modulated. Each of the one or more light sources can be, e.g., a laser or a light-emitting diode. The methods and devices of the disclosure are useful for performing genetic analysis, e.g.Type: ApplicationFiled: August 13, 2013Publication date: March 6, 2014Applicant: IntegenX Inc.Inventors: Ezra Van Gelder, Stephen J. Williams
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Publication number: 20140065689Abstract: The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage may also optionally be preserved, i.e., the absorbed biomolecule is resistant to or resists degradation.Type: ApplicationFiled: March 12, 2013Publication date: March 6, 2014Applicant: IntegenX Inc.Inventors: Michael Hogan, James C. Davis
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Publication number: 20140045704Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.Type: ApplicationFiled: May 2, 2013Publication date: February 13, 2014Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, luliu I. Balga, Allen R. Boronkay, Joanne Horn, Michael Van Nguyen, William D. Nielsen, Mattias Vangbo
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Publication number: 20130344475Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: ApplicationFiled: May 17, 2013Publication date: December 26, 2013Applicant: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga, Allen Boronkay
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Patent number: 8584703Abstract: This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.Type: GrantFiled: November 18, 2010Date of Patent: November 19, 2013Assignee: IntegenX Inc.Inventors: Boris Kobrin, Iuliu I. Blaga, William Nielsen, Shize D. Qi, Ezra Van Gelder
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Patent number: 8562918Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: GrantFiled: December 17, 2012Date of Patent: October 22, 2013Assignee: IntegenX Inc.Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
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Patent number: 8557518Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.Type: GrantFiled: July 28, 2010Date of Patent: October 15, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu I. Blaga, Michael Nguyen, William D. Nielsen, Mattias Vangbo
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Patent number: 8551714Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: February 6, 2012Date of Patent: October 8, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20130224846Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.Type: ApplicationFiled: December 17, 2012Publication date: August 29, 2013Applicant: IntegenX Inc.Inventor: IntegenX Inc.
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Patent number: 8512538Abstract: This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries.Type: GrantFiled: May 23, 2011Date of Patent: August 20, 2013Assignee: IntegenX Inc.Inventors: Lars Majlof, Ezra Van Gelder
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Publication number: 20130203634Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.Type: ApplicationFiled: August 20, 2011Publication date: August 8, 2013Applicant: INTEGENX INC.Inventors: Stevan B. Jovanovich, Seth Stern, Ezra Van Gelder, Mattias Vangbo
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Patent number: 8476063Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: June 15, 2010Date of Patent: July 2, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Publication number: 20130139895Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.Type: ApplicationFiled: August 20, 2011Publication date: June 6, 2013Applicant: INTEGENX INC.Inventor: Mattias Vangbo
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Publication number: 20130115607Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.Type: ApplicationFiled: October 19, 2012Publication date: May 9, 2013Applicant: IntegenX Inc.Inventor: IntegenX Inc.
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Patent number: 8431340Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: October 23, 2009Date of Patent: April 30, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga