Patents Assigned to Integrated DNA Technologies
  • Patent number: 11034989
    Abstract: The invention provides methods for the synthesis of long oligonucleotides, genes and gene fragments. The methods include the manufacture of genes or gene fragments that can be then inserted into a variety of vectors.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: June 15, 2021
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Shawn Allen, Stephen Gunstream, Scott Rose
  • Patent number: 10982273
    Abstract: The invention is directed to methods and kits for performing an RNase H2-mediated cleavage reaction on a sample in an emulsion.
    Type: Grant
    Filed: August 9, 2017
    Date of Patent: April 20, 2021
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Joseph Dobosy, Sarah Jacobi, Richard Owczarzy, Mark Behlke, Jessica Lister
  • Patent number: 10982247
    Abstract: This invention relates to mutant DNA polymerases having an enhanced template discrimination activity compared with the corresponding unmodified DNA polymerase counterparts, wherein the amino acid sequence of the mutant DNA polymerase includes at least one substitution at residue positions structurally and functionally homologous or orthologous positions 783 or 784 of an unmodified Taq DNA polymerase.
    Type: Grant
    Filed: December 21, 2016
    Date of Patent: April 20, 2021
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Mark Aaron Behlke, Joseph A. Walder, Richard Owczarzy, Scott D. Rose, Joseph R. Dobosy, Susan M. Rupp
  • Patent number: 10894979
    Abstract: The invention pertains to construction of next-generation DNA sequencing (NGS) libraries for whole genome sequencing, targeted resequencing, sequencing-based screening assays, metagenomics, or any other application requiring sample preparation for NGS.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: January 19, 2021
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Zachary Zwirko, Yu Zheng, Mirna Jarosz, Caifu Chen, Joseph Walder
  • Patent number: 10886006
    Abstract: The invention can be used to provide a more efficient and less error-prone method of detecting variants in DNA, such as SNPs and indels. The invention also provides a method for performing inexpensive multiplex assays. The invention also provides methods for detection of DNA sequences altered after cleavage by a targetable endonuclease, such as the CRISPR Cas9 protein from the bacterium Streptococcus pyogenes.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: January 5, 2021
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Joseph Dobosy, Caifu Chen, Mark Aaron Behlke, Garrett Richard Rettig
  • Patent number: 10858699
    Abstract: The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: December 8, 2020
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Joseph A. Walder, Joseph Dobosy, Mark Aaron Behlke
  • Patent number: 10838939
    Abstract: Disclosed herein are nucleic acid-based data storage systems and nucleic acid data storage constructs comprising reusable nucleic acid sequences, each representing information carried by a single bit (and, in some embodiments, one or more adjacent bits) within a bit string, and each furthermore representing the position of the single bit within the bit string. Also described are methods for storing data in the nucleic acid-based data storage systems and nucleic acid data storage constructs of the disclosure.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: November 17, 2020
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Joseph Alan Walder, Jeffrey A. Manthey, William E. Martin, III, Shawn Allen
  • Patent number: 10767176
    Abstract: This invention pertains to modified compositions for use in CRISPR systems, and their methods of use. In particular, length-modified and chemically-modified forms of crRNA and tracrRNA are described for use as a reconstituted guide RNA for interaction with Cas9 of CRISPR systems. The resultant length-modified and chemically-modified forms of crRNA and tracrRNA are economical to produce and can be tailored to have unique properties relevant to their biochemical and biological activity in the context of the CRISPR Cas9 endonuclease system.
    Type: Grant
    Filed: October 21, 2016
    Date of Patent: September 8, 2020
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Michael Allen Collingwood, Ashley Mae Jacobi, Garrett Richard Rettig, Mollie Sue Schubert, Mark Aaron Behlke
  • Patent number: 10717978
    Abstract: This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR/Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR/Cas endonuclease system, wherein the CRISPR/Cas endonuclease system displays reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR/Cas endonuclease systems having mutant Cas9 proteins that display reduced off-target editing activity and maintained on-target editing activity relative to a wild-type CRISPR/Cas endonuclease system.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: July 21, 2020
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Christopher Anthony Vakulskas, Michael Allen Collingwood, Garrett Richard Rettig, Mark Aaron Behlke
  • Patent number: 10683542
    Abstract: The invention pertains to construction of next-generation DNA sequencing (NGS) libraries for whole genome sequencing, targeted resequencing, sequencing-based screening assays, metagenomics, or any other application requiring sample preparation for NGS.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: June 16, 2020
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Zachary Zwirko, Yu Zheng, Mirna Jarosz, Caifu Chen, Joseph Walder
  • Patent number: 10653800
    Abstract: The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: May 19, 2020
    Assignees: University of Iowa Research Foundation, Integrated DNA Technologies, Inc.
    Inventors: James O. McNamara, II, Katie R. Flenker, Lingyan Huang, Alexander R. Horswill, Mark A. Behlke, Frank J. Hernandez
  • Patent number: 10266889
    Abstract: The invention is directed to modified oligonucleotide compositions and methods for selectively reducing unwanted nucleic acid contaminants and enriching for desired nucleic acid targets from complex genomic nucleic acid mixtures for sequencing applications. The modified oligonucleotide compositions include one or more modified groups that increase the Tm of the resultant oligonucleotide composition.
    Type: Grant
    Filed: July 3, 2013
    Date of Patent: April 23, 2019
    Assignees: ROCHE FINANCE LTD, INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Mark Aaron Behlke, John Robert Havens, Mirna Jarosz, Zachary Zwirko, Doron Lipson, Frank Soo Juhn
  • Patent number: 10233450
    Abstract: The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: March 19, 2019
    Assignees: CITY OF HOPE, INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: John J. Rossi, Mark A. Behlke, Dongho Kim
  • Patent number: 10227641
    Abstract: Methods and compositions are provided for improving specificity during amplification of a target DNA sequence. The methods and compositions rely upon the use of an RNase H enzyme, a polymerase, and RNase H enzyme-sensitive, blocked-cleavable oligonucleotide primers in the amplification reactions, wherein the reaction mixtures include either an optimized final concentration of a divalent metal salt comprising 2.0 mM or less of free Mg++ cation and/or an optimized final concentration of a non-ionic detergent comprising at least about 0.001% polyethylene glycol hexadecyl ether.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: March 12, 2019
    Assignee: INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: Joseph Alan Walder, Mark Aaron Behlke, Scott D. Rose, Joseph R. Dobosy, Susan M. Rupp
  • Patent number: 10155944
    Abstract: This invention pertains to improved methods for the synthesis of long, double stranded nucleic acid sequences containing difficult to clone or variable regions.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: December 18, 2018
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Shawn Allen, Adam Clore
  • Publication number: 20180340216
    Abstract: The invention pertains to construction of next-generation DNA sequencing (NGS) libraries for whole genome sequencing, targeted resequencing, sequencing-based screening assays, metagenomics, or any other application requiring sample preparation for NGS.
    Type: Application
    Filed: May 25, 2018
    Publication date: November 29, 2018
    Applicant: Integrated DNA Technologies, Inc.
    Inventors: Mirna Jarosz, Madelyn Light, Jiashi Wang, Kristina Giorda
  • Patent number: 10106792
    Abstract: The invention is directed to compositions and methods for selectively reducing the expression of a gene product from a desired target gene in a cell, as well as for treating diseases caused by the expression of the gene. More particularly, the invention is directed to compositions that contain double stranded RNA (“dsRNA”), and methods for preparing them, that are capable of reducing the expression of target genes in eukaryotic cells. The dsRNA has a first oligonucleotide sequence that is between 25 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of at least 19 nucleotides is sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene to trigger the destruction of the target RNA by the RNAi machinery.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: October 23, 2018
    Assignees: CITY OF HOPE, INTEGRATED DNA TECHNOLOGIES, INC.
    Inventors: John J. Rossi, Mark A. Behlke, Dongho Kim
  • Patent number: 10087445
    Abstract: The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: October 2, 2018
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Mark Aaron Behlke, Richard Owczarzy, Yong You, Joseph Alan Walder, Kim Lennox
  • Publication number: 20180223350
    Abstract: This invention pertains to the creation of a complex pool of adapters that contain complementary barcodes to be utilized in next generation sequencing library prep methods and methods of using barcoded adapters for next generation sequencing.
    Type: Application
    Filed: February 7, 2018
    Publication date: August 9, 2018
    Applicant: Integrated DNA Technologies, Inc.
    Inventors: Brendan Galvin, Jiashi Wang
  • Publication number: 20180216177
    Abstract: The invention pertains to construction of next-generation DNA sequencing (NGS) libraries for whole genome sequencing, targeted resequencing, sequencing-based screening assays, metagenomics, or any other application requiring sample preparation for NGS.
    Type: Application
    Filed: January 26, 2018
    Publication date: August 2, 2018
    Applicant: Integrated DNA Technologies, Inc.
    Inventors: Zachary Zwirko, Yu Zheng, Mirna Jarosz, Caifu Chen, Joseph Walder