Abstract: Described are protein constructs of Moloney murine leukemia virus reverse transcriptase (MMLV-RT). In one embodiment the protein construct comprises a Moloney murine leukemia virus reverse transcriptase (MMLV) mature protein sequence; and an expression enhancement sequence at the N-terminus of said MMLV mature protein sequence. The expression enhancement sequence is devised for providing heterologous expression of the protein construct in bacterial cells in absence of chaperone co-expression and/or to provide high-level of expression of the MMLV. In another embodiment the protein construct further comprises a tag/linker combo at the N-terminus of the expression enhancement sequence for assisting in purification of the protein construct. Also described are isolated nucleic acid molecules, vectors and methods for expressing the protein construct in cells. Advantageously these allow large-scale expression of MMLV-RT from cultures of E. coli cells and simplify protocols for purification of MMLV-RT at high purity.

Abstract: Disclosed is a method of maintaining an initial quantity of mRNA vaccine cargo in an EPS container containing dry ice as a passive coolant during shipment from a manufacturing site, wherein the initial quantity of mRNA vaccine cargo is shipped to a first depot and then repackaged into smaller quantities at one or more additional depots en route to the final destinations. Dry ice can be added or ordered in advance to be available at the next depot for the cargo. Indicators show the status of the cargo and whether it has undergone unacceptable temperature or humidity excursions.

Abstract: The invention relates to a smart thermal insulation box, including thermally insulated layers with mitered edges forming a thermally insulated space, and said space surrounded by corrugated material. The box also has externally viewable and operable temperature and humidity sensors, and optionally, a vibration sensor, where the sensors are preferably fixed inside the box to one side. One of the cardboard panels forming the top of the box which overlays the insulation layer has a window configured to overlay the display panel. An audible alert system may also be included to alert users to temperature or humidity excursions.