Abstract: The present invention relates to a method for in vitro producing polynucleotides encoding human germline antibody V-regions. Also disclosed is a library of human germline antibody V-region genes.

Abstract: This invention provides for nonfluorescent, nonenzymatic, chemiluminescent aqueous assays in which the binding of two ligands is determined by a water soluble label system that emits light upon contact with a chemical energy transferring composition.

Abstract: The present invention relates to detection, immobilization, and production of proteolytic antibodies using halogen phosphonate monoester probes, immobilizing reagents, and antigen conjugates.

Abstract: This invention provides origins of replication capable of amplifying nucleic acid at an increased copy number within a cell. In particular, the invention provides origins of replication that amplify plasmid to an increased copy number within a bacterium.

Abstract: The present invention provides a recombinant catalytic polypeptide for cleaving a target protein, the nucleic acid encoding the recombinant catalytic polypeptide, a cell hosting the nucleic acid encoding the recombinant catalytic polypeptide, and a non-human transgenic mammal that is capable of producing a heterologous antibody with proteolytic activity. The invention also provides methods of cleaving a target protein using the recombinant catalytic polypeptides both in vitro and in vivo. The invention further provides a library of recombinant catalytic polypeptides with altered enzymatic activity and a method to alter enzymatic activity of the recombinant catalytic polypeptides.

Abstract: This invention provides origins of replication capable of amplifying nucleic acid at an increased copy number within a cell. In particular, the invention provides origins of replication that amplify plasmid to an increased copy number within a bacterium.