Patents Assigned to Intellicyt Corporation
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Patent number: 10184878Abstract: Methods of evaluating particle attributes in a sample fluid subjected to flow cytometry investigation in a flow cytometer instrument, methods of processing time series signal data traces output by a flow cytometer instrument, and a flow cytometer system are provided. In the methods and systems, data points comprising time series signal data traces corresponding with detection during the flow cytometry investigation of light from the sample fluid in one or more wavelength ranges indicative of the presence of one or more particle attributes in the sample fluid are batch-processed using a batch-specific signal peak threshold determined as a function of a batch-specific noise characteristic to identify signal peaks in the batch of data points indicative of the presence of the one or more particle attributes in the sample fluid.Type: GrantFiled: January 15, 2018Date of Patent: January 22, 2019Assignee: IntelliCyt CorporationInventors: Andrew W. Smolak, Jean-Luc Fraikin, Erica Dawson Tenent, Kathy L. Rowlen
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Patent number: 10161850Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.Type: GrantFiled: March 23, 2016Date of Patent: December 25, 2018Assignee: IntelliCyt CorporationInventors: Michael A. Artinger, Francis Kevin Kohlmeier, Michael W. Olszowy, Tyler Donald Gates
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Patent number: 10101262Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope indicative of an adeno-associated virus viral type or an adenovirus viral type uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.Type: GrantFiled: September 22, 2017Date of Patent: October 16, 2018Assignee: IntelliCyt CorporationInventors: Michael A. Artinger, Francis Kevin Kohlmeier, Michael W. Olszowy, Tyler Donald Gates
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Patent number: 10031061Abstract: The present invention relates to methods that enable improved accuracy for quantitative particle counting in a flowing liquid stream. The methods of the present invention utilize the real-time measurement of flow rates and flow rate control through feedback mechanisms to improve quantification, and this improved quantification translates to more accurate particle counting. In certain embodiments, particles being counted are biological particles in a liquid sample, such as viruses.Type: GrantFiled: May 13, 2009Date of Patent: July 24, 2018Assignee: IntelliCyt CorporationInventors: Kathy L. Rowlen, Matthew Ferris
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Patent number: 9927346Abstract: A flow cytometer system includes a flow cytometer, an autosampler and a system integration structure to accommodate interconnection and interface of the flow cytometer and autosampler for operation together and providing for convenient interface with equipment for handling process liquids.Type: GrantFiled: September 26, 2017Date of Patent: March 27, 2018Assignee: IntelliCyt CorporationInventors: Garrett S. Wilson, Braden L. Smith, Michael A. Artinger, Francis Kevin Kohlmeier, Christopher H. Converse
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Patent number: 9903803Abstract: Methods of evaluating particle attributes in a sample fluid subjected to flow cytometry investigation in a flow cytometer instrument, methods of processing time series signal data traces output by a flow cytometer instrument, and a flow cytometer system are provided. In the methods and systems, data points comprising time series signal data traces corresponding with detection during the flow cytometry investigation of light from the sample fluid in one or more wavelength ranges indicative of the presence of one or more particle attributes in the sample fluid are batch-processed using a batch-specific signal peak threshold determined as a function of a batch-specific noise characteristic to identify signal peaks in the batch of data points indicative of the presence of the one or more particle attributes in the sample fluid.Type: GrantFiled: June 3, 2015Date of Patent: February 27, 2018Assignee: Intellicyt CorporationInventors: Andrew W. Smolak, Jean-Luc Fraikin, Erica Dawson Tenent, Kathy L. Rowlen
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Patent number: 9880085Abstract: A flow cytometer system includes a flow cytometer, an autosampler and a system integration structure to accommodate interconnection and interface of the flow cytometer and autosampler for operation together and providing for convenient interface with equipment for handling process liquids.Type: GrantFiled: March 13, 2015Date of Patent: January 30, 2018Assignee: IntelliCyt CorporationInventors: Garrett S. Wilson, Braden L. Smith, Michael A. Artinger, Francis Kevin Kohlmeier, Christopher H. Converse
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Patent number: 9850979Abstract: A flow cytometer may include a vibration isolation structure on which a flow cytometer optical system assembly is supported when the flow cytometer is in an operational configuration. A shear protection structure may be positioned to protect a vibration isolation structure from damage during handling and shipping. A flow cytometer optical system assembly may include optical component units fixed in position on a support platform with adjustability of one or more optical features in the optical component units. A light-tight dichroic mirror unit may include a rotatably mounted dichroic mirror with a locking mechanism to permit re-setting angular positioning of a dichroic mirror.Type: GrantFiled: June 2, 2015Date of Patent: December 26, 2017Assignee: IntelliCyt CorporationInventors: Kathy L. Rowlen, Erica Dawson Tenent, Garrett S. Wilson, Christopher H. Converse
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Patent number: 9816912Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope indicative of an adeno-associated virus viral type or an adenovirus viral type uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.Type: GrantFiled: March 23, 2016Date of Patent: November 14, 2017Assignee: IntelliCyt CorporationInventors: Michael A. Artinger, Francis Kevin Kohlmeier, Michael W. Olszowy, Tyler Donald Gates
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Patent number: 9012235Abstract: Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer.Type: GrantFiled: August 17, 2012Date of Patent: April 21, 2015Assignee: IntelliCyt CorporationInventors: Linda Trinkle, R. Terry Dunlay, Bruce Edwards, Larry Sklar
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Patent number: 8823943Abstract: One embodiment of the present invention provides for a method for identifying within a single record the location of each of a plurality of samples suspected of containing particles of interest wherein the single record is obtained from a flowing stream of the plurality of samples passing through a particle analyzer. The method comprises introducing into a conduit the plurality of samples suspected of containing particles of interest wherein each ones of the plurality of samples are separated by fluid gaps to produce a plurality of samples separated by fluid gaps and wherein each of the plurality of samples further comprises marker particles. The plurality of samples separated by fluid gaps are flowed through the conduit as a flowing sample stream to a detector of a particle analyzer, which may be a flow cytometer. The particles of interest are detected as the samples pass the detector of the particle analyzer.Type: GrantFiled: May 21, 2010Date of Patent: September 2, 2014Assignee: Intellicyt CorporationInventors: Linda Trinkle, Thomas Duensing
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Publication number: 20140152987Abstract: One embodiment of the present invention provides for a method for identifying within a single record the location of each of a plurality of samples suspected of containing particles of interest wherein the single record is obtained from a flowing stream of the plurality of samples passing through a particle analyzer. The method comprises introducing into a conduit the plurality of samples suspected of containing particles of interest wherein each ones of the plurality of samples are separated by fluid gaps to produce a plurality of samples separated by fluid gaps and wherein each of the plurality of samples further comprises marker particles. The plurality of samples separated by fluid gaps are flowed through the conduit as a flowing sample stream to a detector of a particle analyzer. The particle analyzer is for example a flow cytometer. The particles of interest when present and/or marker particles are detected as the plurality of samples pass the detector of the particle analyzer.Type: ApplicationFiled: February 7, 2014Publication date: June 5, 2014Applicant: Intellicyt CorporationInventors: Linda Trinkle, Thomas Duensing
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Publication number: 20120309635Abstract: Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer.Type: ApplicationFiled: August 17, 2012Publication date: December 6, 2012Applicant: INTELLICYT CORPORATIONInventors: Linda Trinkle, R. Terry Dunlay, Bruce Edwards, Larry Sklar
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Publication number: 20120061584Abstract: One embodiment of the present invention provides for a method for identifying within a single record the location of each of a plurality of samples suspected of containing particles of interest wherein the single record is obtained from a flowing stream of the plurality of samples passing through a particle analyzer. The method comprises introducing into a conduit the plurality of samples suspected of containing particles of interest wherein each ones of the plurality of samples are separated by fluid gaps to produce a plurality of samples separated by fluid gaps and wherein each of the plurality of samples further comprises marker particles. The plurality of samples separated by fluid gaps are flowed through the conduit as a flowing sample stream to a detector of a particle analyzer, which may be a flow cytometer. The particles of interest are detected as the samples pass the detector of the particle analyzer.Type: ApplicationFiled: May 21, 2010Publication date: March 15, 2012Applicant: Intellicyt CorporationInventors: Linda Trinkle, Thomas Duensing
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Patent number: D722515Type: GrantFiled: July 3, 2013Date of Patent: February 17, 2015Assignee: Intellicyt CorporationInventor: Joseph M. Zock
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Patent number: D737703Type: GrantFiled: February 17, 2015Date of Patent: September 1, 2015Assignee: Intellicyt CorporationInventor: Joseph M. Zock
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Patent number: D787701Type: GrantFiled: March 21, 2014Date of Patent: May 23, 2017Assignee: Intellicyt CorporationInventors: Garrett S. Wilson, Braden L. Smith, Michael A. Artinger