Patents Assigned to Intelligent Bio-Systems, Inc.
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Patent number: 10329611Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: March 5, 2018Date of Patent: June 25, 2019Assignee: Intelligent Bio-Systems, Inc.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 10017816Abstract: The invention relates to methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, and in particular, sequences that contain consecutive repeats of a particular base. For example, a consecutive repeat of a particular base may be identified by a charged ion detection (e.g., hydrogen ion detection).Type: GrantFiled: April 11, 2016Date of Patent: July 10, 2018Assignee: INTELLIGENT BIO-SYSTEMS, INC.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 9926595Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: March 22, 2017Date of Patent: March 27, 2018Assignee: INTELLIGENT BIO-SYSTEMS, INC.Inventors: Jerzy Olejnik, Evan Guggenheim, Visalakshi Meyyappan
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Patent number: 9909174Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: July 20, 2016Date of Patent: March 6, 2018Assignee: Intelligent Bio-Systems, Inc.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 9879309Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: April 5, 2013Date of Patent: January 30, 2018Assignee: INTELLIGENT BIO-SYSTEMS, INC.Inventors: Steven Gordon, Phillip A Veatch, Jerzy Olejnik
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Patent number: 9828632Abstract: An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.Type: GrantFiled: November 12, 2014Date of Patent: November 28, 2017Assignee: INTELLIGENT BIO-SYSTEMS, INC.Inventors: Steven Gordon, Steven Auger, Alan Schinazi
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Patent number: 9644237Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: May 2, 2016Date of Patent: May 9, 2017Assignee: INTELLIGENT BIO-SYSTEMS, Inc.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 9605301Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: August 11, 2014Date of Patent: March 28, 2017Assignee: Intelligent Bio-Systems, Inc.Inventors: Jerzy Olejnik, Evan Guggenheim, Visalakshi Meyyappan
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Patent number: 9434989Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: April 20, 2015Date of Patent: September 6, 2016Assignee: INTELLIGENT BIO-SYSTEMS, INC.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 9322050Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: July 25, 2013Date of Patent: April 26, 2016Assignee: Intelligent Bio-Systems, Inc.Inventor: Jerzy Olejnik
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Patent number: 9309569Abstract: The invention provides methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, including sequences with one or more homopolymer regions. The methods of the invention include improvements so as to accurately identify sequences, including the difficult homopolymer sequences that are encountered during nucleotide sequencing, such as pyrosequencing.Type: GrantFiled: August 18, 2011Date of Patent: April 12, 2016Assignee: Intelligent Bio-Systems, Inc.Inventors: Steven Gordon, Jerzy Olejnik
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Patent number: 9146248Abstract: A method for operating a nucleic acid sequencing instrument with movable flow cells. The method includes providing a flow cell having a flow path with an inlet port and an outlet port and filling the flow path, from the inlet port to the outlet port, with a first liquid reagent. The method also includes providing a station block having an inlet passage and an outlet passage, mounting the flow cell on the station block with the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage, introducing a gaseous bubble into the inlet port, conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent, and removing the flow cell from the station block.Type: GrantFiled: March 14, 2013Date of Patent: September 29, 2015Assignee: Intelligent Bio-Systems, Inc.Inventors: Thomas Hagerott, Edmund W. Golaski
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Patent number: 8900810Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.Type: GrantFiled: March 6, 2013Date of Patent: December 2, 2014Assignee: Intelligent Bio Systems, Inc.Inventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik
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Publication number: 20140342363Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: ApplicationFiled: May 20, 2013Publication date: November 20, 2014Applicant: Intelligent Bio-Systems, Inc.Inventors: Steven Gordon, Phillip A. Veatch
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Patent number: 8883999Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: March 17, 2009Date of Patent: November 11, 2014Assignee: Intelligent Bio Systems, Inc.Inventors: Jerzy Olejnik, Evan Guggenheim, Visalakshi Visalakshi
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Publication number: 20140259607Abstract: A method for operating a nucleic acid sequencing instrument with movable flow cells. The method includes providing a flow cell having a flow path with an inlet port and an outlet port and filling the flow path, from the inlet port to the outlet port, with a first liquid reagent. The method also includes providing a station block having an inlet passage and an outlet passage, mounting the flow cell on the station block with the inlet port in fluid communication with the inlet passage and the outlet port in fluid communication with the outlet passage, introducing a gaseous bubble into the inlet port, conveying a second liquid reagent from the inlet passage into the inlet port to move the gaseous bubble through the flow path and into the outlet passage and fill the flow passage with the second liquid reagent, and removing the flow cell from the station block.Type: ApplicationFiled: March 14, 2013Publication date: September 18, 2014Applicant: Intelligent Bio-Systems, Inc.Inventors: THOMAS HAGEROTT, Edmund W. Golaski
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Publication number: 20140267669Abstract: Imagers and alignment methods for use by imagers imaging deoxyribonucleic acid (DNA) fragments on a flow cell are disclosed. The imagers capture intensity values at DNA fragment bead locations in tiles with each tile having a reference location in the flow cell. Flow cells may be aligned by obtaining a dark field image of each tile during a first imaging session, identifying dark field constellations of bead locations within two separate tiles during the first imaging session, identifying corresponding constellations during a second imaging session, altering the reference location of at least one tile during the second imaging session to correct for a linear offset in the corresponding constellations, and applying at least one correction factor for reading out intensity values from the imager for the bead locations in the flow cell to correct for an angular offset determined from offsets in the corresponding constellations.Type: ApplicationFiled: March 15, 2013Publication date: September 18, 2014Applicant: Intelligent Bio-Systems, Inc.Inventors: DAVID RAY STOOPS, Phillip Alan Veatch, Steven Jeffrey Gordon
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Patent number: 8741815Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.Type: GrantFiled: February 12, 2009Date of Patent: June 3, 2014Assignee: Intelligent Bio Systems, Inc.Inventors: Steven Gordon, Daniel Stetson
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Patent number: 8623598Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.Type: GrantFiled: March 17, 2009Date of Patent: January 7, 2014Assignee: Intelligent Bio Systems, Inc.Inventors: Jerzy Olejnik, Evan Guggenheim, Visalakshi Visalakshi
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Patent number: 8481259Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.Type: GrantFiled: March 8, 2010Date of Patent: July 9, 2013Assignee: Intelligent Bio-Systems, Inc.Inventors: Steven Gordon, Thomas Hagerott, Edmund Golaski, Jerzy Olejnik