Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.
Type:
Application
Filed:
June 11, 2010
Publication date:
February 24, 2011
Applicants:
Inter-University Research Institute Corporation, Research Organization of Information and systems, KYOWA HAKKO KIRIN CO., LTD.
Abstract: A transmitter sets a time length, determines a time point at which phases of frequency signals coincide within a range of the time length, defines a determined time point as a time reference point, aligns the phases of the frequency signals based on the time reference point, combines phase-aligned frequency, and transmits combined frequency signals to a receiver by the time length as the communication signals. The receiver receives the communication signals, extracts the phases of the frequency signals, obtains a time point at which the phases coincide, and determines an obtained time point as the time reference point.
Type:
Grant
Filed:
October 18, 2007
Date of Patent:
February 1, 2011
Assignee:
Inter-University Research Institute Corporation/Research Organization of Information and Systems