Abstract: The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.

Abstract: In order to disclose an emission allowance for each product and allow its trading on a product unit basis, the present invention manages, in a management server requesting execution of a settlement process to settlement servers which make settlement of the emission allowance, weight information of the emission allowance and identification information of a non-contact IC medium in association with each other and stores, in databases provided in the settlement servers, weight information of an emission allowance on a seller's side, seller's account information, weight information of an emission allowance on a buyer's side, and buyer's account information.

Abstract: This invention relates to a method for producing a protein of interest, comprising introducing a protein expression vector which comprises a gene fragment a gene fragment comprising a DNA encoding a protein of interest and a selectable marker gene and transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between a pair of the transposon sequences, into a chromosome of the mammalian cell to obtain a mammalian cell capable of expressing the protein of interest; and suspension-culturing the mammalian cell; and a suspension mammalian cell capable of expressing the protein of interest.

Abstract: A transmitter sets a time length, determines a time point at which phases of frequency signals coincide within a range of the time length, defines a determined time point as a time reference point, aligns the phases of the frequency signals based on the time reference point, combines phase-aligned frequency, and transmits combined frequency signals to a receiver by the time length as the communication signals. The receiver receives the communication signals, extracts the phases of the frequency signals, obtains a time point at which the phases coincide, and determines an obtained time point as the time reference point.