Abstract: A method of recovering functional human leukocytes from blood filters used to deplete leukocyte content from leukocyte-containing blood-cell suspensions is provided. More particularly, this method involves isolating leukocytes from filters which have been used to purify red blood cells or platelets in isolation from leukocytes by back-flushing the used filters with a hemolysis solution, such as cold ammonium chloride, and collecting the functional human leukocytes.
Type:
Grant
Filed:
December 22, 1997
Date of Patent:
November 23, 1999
Assignee:
Interferon Science, Inc.
Inventors:
Abbas Rashidbaigi, Mei-June Liao, Ji Hua, Maninder Sidhu