Abstract: A device for the horizontal electroblotting of electrophoretically transferred material comprising a fluid tight container having a floor, a lid and side walls extending between the floor and the lid for defining a chamber, a support assembly located in the chamber for supporting the material to be electroblotted, a first electrode provided below the support assembly and a second electrode provided above the support assembly and a barrier provided in the chamber between the first electrode and the support assembly for diverting bubbles produced by the first electrode so as to prevent the bubbles from accumulating on the support assembly during the electroblotting of the electrophoretically transferred material.
Abstract: A rapid and effective method for electroblotting is provided whereby an electrophoretically resolved material in a gelatin sheet is quickly and efficiently transferred to a membrane with high pattern definition and resolution.
Abstract: This application discloses a horizontal gel slab electrophoresis device comprised of a base containing two buffer chambers having a central divider means positioned below a horizontal self. The horizontal shaft is provided with means for cooling. A movable gel tray is positioned on top of the shelf for containing the gel slab and samples to be analyzed. An electrode is positioned in each buffer chamber adjacent to the central divider means. A valve means is positioned in the central divider means for controlling fluid flow between the buffer chambers. In addition, a port is positioned in each buffer chamber for feeding fluids into each of the buffer chambers.The device is useful in the electrophoresis analysis of samples contained in the gel slab within the movable gel tray.
Type:
Grant
Filed:
February 27, 1984
Date of Patent:
May 13, 1986
Assignee:
International Biotechnologies, Inc.
Inventors:
John H. Kreisher, Hal D. Belle Isle, Charles A. Nalbantian
Abstract: A simplified electroelution receptacle designed to receive and process a plurality of small biological samples simultaneously facilitating efficient recovery of biological particles in a highly concentrated manner with a minimum of undesirable liquid.
Abstract: An electrophoresis apparatus including a mold for forming a vertically disposed electrophoresis gel slab where the slab is of uniformly increasing thickness from top to bottom and method of fabricating the mold and the gel slab.
Type:
Grant
Filed:
January 14, 1985
Date of Patent:
March 18, 1986
Assignee:
International Biotechnologies, Inc.
Inventors:
John H. Kreisher, Charles A. Nalbantian
Abstract: A novel receptacle for removing charged particles from a gel matrix or gel slice containing biological substances such as nucleic acids, proteins, carbohydrates and the like by electrophoresis procedures operable to recover and collect the particles in concentrated fashion with a minimum of undesirable fluid.
Type:
Grant
Filed:
November 5, 1984
Date of Patent:
March 18, 1986
Assignee:
International Biotechnologies, Inc.
Inventors:
Lawrence J. Peck, John H. Kreisher, Alan L. Walker