Abstract: A diagnostic cell test and kit for detecting human chorionic gonadotropin as evidence of cancer. The subject's cells undergoing examination are contacted with antiserum specific to the beta-subunit of human chorionic gonadotropin, the antiserum having been raised in a host animal. The treated cells are divided into two portions. The portions are treated with antiserum to the gamma globulin of the host animal. The antiserum to the gamma globulin used in treating the first portion is conjugated to fluorescin, and the antiserum used in contacting the second portion is conjugated to peroxidase. A positive test for cancer is concluded only when both tests on the two portions of the cells are positive.
Type:
Grant
Filed:
October 6, 1978
Date of Patent:
October 14, 1980
Assignee:
International Radioimmune Systems, Inc.
Inventors:
Hernan F. Acevedo, Malcolm Slifkin, Milton H. Dalbow
Abstract: A diagnostic cell test and kit for detecting human chorionic gonadotropin as evidence of cancer. The subject's cells undergoing examination are contacted with artiserum specific to the beta-subunit of human chorionic gonadotropin, the antiserum having been raised in a host animal. The treated cells are divided into two portions. The portions are treated with antiserum to the gamma globulin of the host animal. The antiserum to the gamma globulin used in treating the first portion is conjugated to fluorescein and the antiserum used in contacting the second portion is conjugated to peroxidase. A positive test for cancer is concluded only when both tests on the two portions of the cells are positive.
Type:
Grant
Filed:
April 19, 1976
Date of Patent:
March 13, 1979
Assignee:
International Radioimmune Systems, Inc.
Inventors:
Hernan F. Acevedo, Malcolm Slifkin, Milton H. Dalbow
Abstract: Malignant neoplastic cells synthesize and secrete human chorionic gonadotropin into the circulatory system, where the glycoprotein hormone is collected or adsorbed by the host's lymphocytes. This application discloses a clinical diagnostic test and kit for determining the presence of human chorionic gonadotropin in blood. Where the test is positive in a man or a non-pregnant woman cancer is present in the host.
Type:
Grant
Filed:
April 19, 1976
Date of Patent:
September 26, 1978
Assignee:
International Radioimmune Systems, Inc.
Inventors:
Milton H. Dalbow, Hernan F. Acevedo, Malcolm Slifkin