Patents Assigned to International Reagents Corporation
-
Patent number: 6986998Abstract: There is provided a method for a selective assay of component, particularly cholesterol, in very low-density lipoprotein (VLDL) which is one of serum lipoproteins. In the assay, an enzymatic reaction of lipoprotein lipase (LPL) or cholesterol esterase (CE) which well reacts with high-density lipoprotein (HDL) and VLDL is carried out at least in the presence of calixarene or a salt thereof. It is also carried out in the presence of one or more substance(s) selected from albumin and basic amino acids in addition to calixarene or a salt thereof.Type: GrantFiled: June 5, 2001Date of Patent: January 17, 2006Assignee: International Reagents CorporationInventors: Koji Kishi, Tsutomu Kakuyama, Koji Ochiai
-
Publication number: 20030228316Abstract: The object of the present invention is to provide a method for more accurately assaying the enzyme activities of mCK isozymes and a method for separately assaying the enzyme activities of CK isozymes by separately assaying the enzyme activities of ubiquitous mCK (umCK) and sarcomeric mCK (smCK).Type: ApplicationFiled: April 29, 2003Publication date: December 11, 2003Applicant: INTERNATIONAL REAGENTS CORPORATIONInventors: Yasushi Shirahase, Tadahiro Kajita, Koji Kishi, Kazuaki Yamashita
-
Patent number: 6639053Abstract: The present invention provides a gene encoding an RNA polymerase which plays an important role in the reproduction of hepatitis C virus, and a method of screening using this gene or this RNA polymerase protein, thereby allowing easy performance of screening for substances which inhibit the RNA polymerase playing an important role in HCV reproduction.Type: GrantFiled: December 21, 2001Date of Patent: October 28, 2003Assignees: International Reagents Corporation, Chugai Seiyaku Kabushiki KaishaInventors: Tetsuya Toyoda, Michinori Kohara, Kyoko Kohara, Kazuhiro Higashi, Masayuki Tsuchiya
-
Publication number: 20030143631Abstract: A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria-localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor are provided.Type: ApplicationFiled: February 11, 2003Publication date: July 31, 2003Applicant: INTERNATIONAL REAGENTS CORPORATIONInventors: Yasushi Shirahase, Tadahiro Kajita, Tadashi Hoshino
-
Patent number: 6586194Abstract: A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor.Type: GrantFiled: September 19, 2000Date of Patent: July 1, 2003Assignee: International Reagents CorporationInventors: Yasushi Shirahase, Tadahiro Kajita, Tadashi Hoshino
-
Publication number: 20020151646Abstract: This invention provides a novel thromboplastin reagent having high measurement sensitivity. Thus, an effective amount of amino acid or derivative thereof having such a function that an ISI (international sensitivity index) of a composition of thromboplastin showing an ISI of more than 1.0 is made nearer 1.0 is added whereby a thromboplastin having a high measurement sensitivity is provided. To be more specific, sodium glutamate is added in an effective amount.Type: ApplicationFiled: February 5, 2002Publication date: October 17, 2002Applicant: INTERNATIONAL REAGENTS CORPORATIONInventors: Norihiro Kikukawa, Masahiro Okuda
-
Patent number: 6242208Abstract: By a process comprising inhibiting LDH2, LDH3, LDH4 and LDH5 in a sample with a protease in the presence of a protein-denaturating agent and then determining LDH1 remaining uninhibited, LDH1 in the sample is easily determined.Type: GrantFiled: May 2, 1994Date of Patent: June 5, 2001Assignee: International Reagents CorporationInventors: Yasushi Shirahase, Kenji Isshiki, Yoshifumi Watazu
-
Patent number: 6114134Abstract: A method for assaying biological specimens for substances contained in the components of each specimen by the use of one or more kinds of calixarenes; and a reagent comprising one or more kinds of calixarenes. The method utilizes complexes formed by calixarenes and the components of biological specimens, and makes it possible to assay biological specimens for substances contained in the components of each specimen, for example, for cholesterol contained in high-density lipoprotein (HDL), without preliminary separation from the other components of the biological specimen. The method can be conducted by easy and simple operations and lessen measurement errors or problems caused by man, and permits continuous measurement with general-purpose automatic analyzing apparatuses and multi-channel measurement together with other test items.Type: GrantFiled: December 2, 1999Date of Patent: September 5, 2000Assignee: International Reagents CorporationInventors: Koji Kishi, Tsutomu Kakuyama, Yasushi Shirahase, Yoshifumi Watazu
-
Patent number: 5871904Abstract: This invention concerns a monoclonal antibody having binding specificity for an antigenic determinant site on core structural protein from Non-A,Non-B hepatitis virus (NANBV); a hybridoma cell line capable of producing the monoclonal antibody; a process for the preparation of the monoclonal antibody; an immunoassay of NANBV-related antigens by use of the monoclonal antibody; and a test kit for use in the immunoassay. The preferred monoclonal antibody is 5E3, 5F11, 515S or 1080S. The monoclonal antibody can specifically recognize the NANBV core structural protein in sera from patients with Non-A,Non-B hepatitis thereby being served extensively as an antibody in various immunological reagents for definitive diagnosis of Non-A, Non-B hepatitis.Type: GrantFiled: July 11, 1995Date of Patent: February 16, 1999Assignees: The Tokyo Metropolitan Institute of Medical Science, International Reagent Corporation, Tonen CorporationInventors: Tomiko Kashiwakuma, Shintaro Yagi, Akira Hasegawa, Tadahiro Kajita, Yohsuke Ohta, Hiroyuki Mori
-
Patent number: 5767247Abstract: Using human annexin-V or human annexin-V plus dog annexin-V as antigen(s), hybridoma cell lines are prepared which are capable of producing anti-annexin-V monoclonal antibodies having a binding specificity to antigenic determinant site on annexin-V as antigenic protein and belonging to immunoglobulin G class. By the hybridoma cell lines are produced the anti-annexin-V monoclonal antibodies, with which a diagnostic agent is provided for diagnosis of myocardial infarction and angina pectoris.Type: GrantFiled: October 22, 1996Date of Patent: June 16, 1998Assignees: Noboru Kaneko, International Reagents CorporationInventors: Noboru Kaneko, Ryuko Matsuda, Tadahiro Kajita, Yohsuke Ohta
-
Patent number: 5122455Abstract: A glutamic-oxaloacetic transaminase (GOT) isozyme assay, which comprises selectively inhibiting s-GOT in a sample such as serum or plasma by proteinase K, optionally an excess amount of proteinase K by a protease inhibitor and then determining m-GOT remaining intact, is described.Type: GrantFiled: February 23, 1990Date of Patent: June 16, 1992Assignees: International Reagents Corporation, Amano Pharmaceutical Co., Ltd.Inventors: Yasushi Shirahase, Yoshifumi Watazu
-
Patent number: D472975Type: GrantFiled: March 28, 2002Date of Patent: April 8, 2003Assignee: International Reagents CorporationInventors: Hitomi Iori, Nobuya Kajiwara, Koji Ochiai
-
Patent number: D474271Type: GrantFiled: March 28, 2002Date of Patent: May 6, 2003Assignee: International Reagents CorporationInventors: Hitomi Iori, Nobuya Kajiwara, Koji Ochiai