Patents Assigned to Intrinsic Bioprobes, Inc.
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Publication number: 20070243636Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: March 28, 2007Publication date: October 18, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20070243635Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: March 28, 2007Publication date: October 18, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20070166766Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: March 28, 2007Publication date: July 19, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20070166767Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: March 30, 2007Publication date: July 19, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20070128663Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: February 6, 2007Publication date: June 7, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20070128734Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.Type: ApplicationFiled: February 6, 2007Publication date: June 7, 2007Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20060270062Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.Type: ApplicationFiled: August 4, 2006Publication date: November 30, 2006Applicant: INTRINSIC BIOPROBES, INC.Inventors: Randall Nelson, Peter Williams, Jennifer Krone
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Publication number: 20060246514Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.Type: ApplicationFiled: July 24, 2006Publication date: November 2, 2006Applicant: INTRINSIC BIOPROBES, INC.Inventors: Randall Nelson, Peter Williams, Jennifer Krone
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Patent number: 7087164Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.Type: GrantFiled: August 16, 2004Date of Patent: August 8, 2006Assignee: Intrinsic Bioprobes, Inc.Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
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Patent number: 7087163Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.Type: GrantFiled: August 16, 2004Date of Patent: August 8, 2006Assignee: Intrinsic Bioprobes, Inc.Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
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Patent number: 7087165Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.Type: GrantFiled: August 16, 2004Date of Patent: August 8, 2006Assignee: Intrinsic Bioprobes, Inc.Inventors: Kemmons A Tubbs, Karl F. Gruber, Randall W. Nelson
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Patent number: 7083724Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.Type: GrantFiled: August 16, 2004Date of Patent: August 1, 2006Assignee: Intrinsic Bioprobes, Inc.Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
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Patent number: 7083723Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.Type: GrantFiled: August 16, 2004Date of Patent: August 1, 2006Assignee: Intrinsic Bioprobes, Inc.Inventors: Kemmons A. Tubbs, Karl F. Gruber, Randall W. Nelson
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Publication number: 20060127948Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.Type: ApplicationFiled: October 24, 2005Publication date: June 15, 2006Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Publication number: 20060121533Abstract: Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins present in complex biological fluids or extracts. Pipettor tips containing porous solid supports that are covalently derivatized with affinity ligand and used to extract specific proteins and their variants from various biological fluids. Nonspecifically bound compounds are rinsed from the extraction devices using a series of buffer and water rinses, after which the wild type protein (and/or its variants) are eluted directly onto a target in preparation for analysis such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Mass spectrometry of the eluted sample then follows with the retained proteins identified via accurate molecular mass determination.Type: ApplicationFiled: October 24, 2005Publication date: June 8, 2006Applicant: INTRINSIC BIOPROBES, INC.Inventors: Urban Kiernan, Eric Niederkofler, Kemmons Tubbs, Dobrin Nedelkov, Randall Nelson
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Patent number: 6974704Abstract: Rapid mass spectrometric immunoassay methods for detecting and/or quantifying antibody and antigen analytes utilizing affinity capture to isolate the analytes and internal reference species (for quantification) followed by mass spectrometric analysis of the isolated analyte/internal reference species. Quantification is obtained by normalizing and calibrating obtained mass spectrum against the mass spectrum obtained for an antibody/antigen of known concentration.Type: GrantFiled: March 14, 2001Date of Patent: December 13, 2005Assignee: Intrinsic Bioprobes, Inc.Inventors: Randall W. Nelson, Peter Williams, Jennifer Reeve Krone
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Patent number: 6887713Abstract: The present invention is directed to a bioactive probe or chip (BC) that allows for the isolation of analytes, such as biomolecules, followed by modification or bioreaction on these said analytes. More specifically, the present invention relates to various methods and apparatuses that include the BC and further include characterization and identification technologies, such as Bioactive Chip Mass Spectrometry (BCMS). Within the context of the present invention, BC provides a method and device for the capture and subsequent modifying, such as digestion or derivatization, of an analyte. Further, real-time information regarding a variety of molecular interactions may be provided by techniques such as interaction analysis (IA). Finally, the variety of molecules are localized and concentrated thereby aiding in the identification and/or quantification of the molecules by techniques such as mass spectroscopy. In one embodiment, a method for performing the modification, or bioreaction, of biomolecules is disclosed.Type: GrantFiled: February 4, 2003Date of Patent: May 3, 2005Assignee: Intrinsic Bioprobes, Inc.Inventors: Randall W. Nelson, Dobrin Nedelkov
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Patent number: 6569383Abstract: The present invention is directed to a bioactive probe or chip (BC) that allows for the isolation of analytes, such as biomolecules, followed by modification or bioreaction on these said analytes. More specifically, the present invention relates to various methods and apparatuses that include the BC and further include characterization and identification technologies, such as Bioactive Chip Mass Spectrometry (BCMS). Within the context of the present invention, BC provides a method and device for the capture and subsequent modifying, such as digestion or derivatization, of an analyte. Further, real-time information regarding a variety of molecular interactions may be provided by techniques such as interaction analysis (IA). Finally, the variety of molecules are localized and concentrated thereby aiding in the identification and/or quantification of the molecules by techniques such as mass spectroscopy. In one embodiment, a method for performing the modification, or bioreaction, of biomolecules is disclosed.Type: GrantFiled: March 11, 2000Date of Patent: May 27, 2003Assignee: Intrinsic Bioprobes, Inc.Inventors: Randall W. Nelson, Dobrin Nedelkov